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. 2005 Jul 19;102(30):10460–10465. doi: 10.1073/pnas.0504249102

Fig. 5.

Fig. 5.

Pateamine inhibits cap-dependent protein synthesis in vivo. (A) Dose-response experiment of pateamine on cells transfected with pcDNA/Ren/HCV/FF. HeLa cells were incubated with the indicated concentrations of pateamine for 10 h, at which time luciferase activity was measured in cell extracts. The relative luciferase activity was determined by comparing to the activity obtained in control cells exposed to DMSO. The average of two experiments is presented with the error of the mean. (B) Northern blot of RNA isolated from cells transfected with pcDNA/Ren/HCV/FF. After transfection, cells were incubated with the indicated concentrations of pateamine. RNA was isolated, fractionated on a 1.2% agarose/formaldehyde gel, and transferred to Hybond N+ (Amersham Pharmacia Biosciences). The blot was then probed with radiolabeled cDNA fragments to Ren/HCV/FF and GAPDH (position of migration indicated). Probes were produced with the Readiprime kit using the manufacturer's recommendations (Amersham Pharmacia).