Skip to main content
. 2002 Jun;13(6):2069–2079. doi: 10.1091/mbc.01-12-0569

Figure 7.

Figure 7

Quantitative analysis of the colocalization between specific chromosomes and stress-induces SNBs. Heat-shocked HeLa cells were independently hybridized to biotinylated probes the for heterochromatic region of HSA9 (pHuR98), HSA12 (pBR12), HSA15 (pMC15), and chromosome X (pDMX1). Cells were costained with anti-HAP polyclonal antibodies to detect stress-induced SNBs. The distribution of HAP and of the biotinylated probe was revealed by indirect immunofluorescence with a rhodamine-conjugated goat anti-rabbit antibody and with FITC-conjugated avidin. Confocal laser microscopy images were taken, and for each chromosome, we counted the number hybridization signals colocalizing with stress bodies. (A) Colocalizing signals in cells with one or two stress-induced SNBs. We analyzed 68 cells (25 of which had a single body) stained for HSA9, 17 cells (8 with one body) stained for HSA12, 15 cells (7 with one body) stained for HSA15, and 16 cells (7 with one body) stained for chromosome X. (B) Cells with more than two stress-induced SNBs were analyzed as in A. We analyzed 211 cells for HSA9, 47 cell for HSA12, 43 cells for HSA15, and 80 cells for chromosome X. ▪, HSA9. ▴, HSA12. ●, HSA15. ○ and dashed line, chromosome X.