Table 2.

Reversed-Phase Liquid Chromatography (RPLC) Gradient.

Time (min)	% MPB
0.0	10
0.5	10
2.0	30
14.0	95
14.5	100
16.5	100
16.5–22.5	10

These parameters are consistent with analysis of PFAS in extracted environmental and clinical samples as adapted from an Agilent application note for PFAS analyses³⁷.