Figure 1.

Molecular characterization of transgenic lines. A, Schematic representation of the transformed chloroplast genome and the pLDR-5′UTR-phaA cassette. Annealing sites for the primer pairs and expected sizes of PCR products are shown. BamHI restriction sites, DNA fragment produced after restriction digestion, and the phaA probe used in Southern-blot analyses are shown. B, The map shows the wild-type chloroplast genome, restriction enzyme sites used for Southern-blot analysis, expected fragment sizes after digestion, and probing with the flanking sequence.