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A, Deduced amino acid sequence of the NtPPME1 cDNA clone. Arrow indicates the predicted SP cleavage site. The Cys residues that are conserved between the pro-region and the PMEI from kiwi fruit are in bold. The catalytically important PME residues (Asp-365, Asp-386, and Arg-454) are underlined. B, Schematic representation indicates the pre-pro-PME that is encoded by NtPPME1. The SP used in the various experiments comprised amino acids 1 to 40 of the deduced NtPPME1 protein sequence.
