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Localization of GFP-tagged NtPPME1 proteins. A, Chimeric gene constructs used for expression in N. tabacum pollen tubes, all under the control of the Lat52 promoter. Expression profiles of NtPPME1-GFP (B), SP-pro-region-GFP (C), and SP-PME-domain-GFP (D) are shown. For each expression profile, a wide-field (I) and a median plane confocal (II) image are shown. Scale bar = 10 μm.
