Table I.
Purification Steps | Activity | Total Protein | Specific Activity | Purification-fold | Yield |
---|---|---|---|---|---|
units | mg | units/mg | % | ||
Sephadex G-200 | 5.45 | 15.59000 | 0.4 | 1.0 | 100 |
Poros HQ | 3.33 | 5.37000 | 0.6 | 1.8 | 61.09 |
Dialysis | 3.12 | 3.71000 | 0.8 | 2.4 | 57.27 |
Poros HS | 0.80 | 0.06530 | 12.3 | 35.0 | 14.67 |
Superdex 200 | 0.07 | 0.00079 | 82.5 | 236.0 | 1.20 |