Figure 2.

Screening of the knockout library of His kinases for survival of Synechocystis cells after heat shock. Cells of individual mutant lines were inoculated separately on plates of agar-solidified BG-11 medium (Stanier et al., 1971) and incubated at 30°C for 3 d. After heat shock at 48°C for either 2 or 3 h, as indicated, plates were incubated at 30°C for a further 7 d. Among the 41 lines with mutation in individual His kinases, only ΔHik34 cells survived.