Table III.
DNA microarray analysis of changes in the genome-wide expression of genes in response to heat shock at 44°C for 20 and 60 min in wild-type and ΔHik34 mutant cells
Genes whose expression was enhanced more than 6-fold in wild-type cells at 20 min are listed. The listed values (ratios of levels of transcripts) were calculated from two to four independent experiments. Genes marked by the same Greek letter are located in tandem on the Synechocystis genome. An apostrophe indicates localization on opposite strands of DNA.
| Gene ID
|
Gene
|
Product
|
Ratio (44°C/34°C, Value ± sd)
|
|||
|---|---|---|---|---|---|---|
| Wild Type
|
ΔHik34
|
|||||
| 20 min | 60 min | 20 min | 60 min | |||
| slr1641α | clpB1 | ClpB protein | 92.5 ± 9.1 | 1.9 ± 0.1 | 74.9 ± 6.4 | 11.1 ± 0.2 |
| sll1514β | hspA | Small HSP | 68.1 ± 2.9 | 21.6 ± 0.1 | 50.7 ± 3.7 | 50.7 ± 1.9 |
| slr2075γ | groES | 10-kD chaperonin | 44.7 ± 2.3 | 15.5 ± 1.0 | 26.9 ± 1.2 | 10.0 ± 0.6 |
| sll0416 | groEL2 | 60-kD chaperonin 2 | 42.1 ± 7.7 | 6.5 ± 0.2 | 14.7 ± 0.1 | 11.5 ± 0.4 |
| slr0093δ | dnaJ | HSP 40 | 36.9 ± 3.6 | 2.2 ± 0.0 | 39.6 ± 0.9 | 3.3 ± 0.2 |
| sll0430 | htpG | HSP 90 | 34.6 ± 5.6 | 3.7 ± 0.2 | 6.3 ± 0.2 | 13.1 ± 0.1 |
| slr1674 | Protein of unknown function | 28.3 ± 0.8 | 1.7 ± 0.1 | 17.3 ± 1.3 | 10.6 ± 0.9 | |
| slr0095δ | O-Methyltransferase | 24.4 ± 4.6 | 0.9 ± 0.1 | 16.4 ± 0.7 | 1.6 ± 0.0 | |
| sll0306 | sigB | RNA polymerase group 2 σ factor | 22.9 ± 0.7 | 1.8 ± 0.2 | 10.9 ± 0.3 | 8.8 ± 0.8 |
| slr1963 | Water-soluble carotenoid protein | 22.8 ± 0.0 | 1.2 ± 0.0 | 2.5 ± 0.1 | 3.4 ± 0.2 | |
| sll0170 | dnaK2 | HSP 70 | 22.3 ± 1.5 | 2.1 ± 0.1 | 13.7 ± 2.7 | 5.3 ± 0.2 |
| sll0528 | Protein of unknown function | 19.5 ± 0.9 | 1.4 ± 0.1 | 13.7 ± 0.8 | 5.3 ± 0.7 | |
| slr1285 | hik34 | His kinase 34 | 18.9 ± 2.6 | 0.6 ± 0.1 | – | – |
| ssl2971α' | Protein of unknown function | 15.1 ± 1.7 | 1.0 ± 0.1 | 15.1 ± 1.9 | 3.2 ± 0.6 | |
| sll0939ɛ | Protein of unknown function | 11.9 ± 0.2 | 0.7 ± 0.1 | 9.1 ± 0.1 | 1.7 ± 0.4 | |
| sll0846 | Protein of unknown function | 11.7 ± 3.0 | 1.8 ± 0.2 | 8.8 ± 0.3 | 5.9 ± 0.6 | |
| sll1621 | AhpC/TSA family protein | 11.0 ± 0.6 | 3.8 ± 0.2 | 12.1 ± 0.4 | 3.2 ± 0.2 | |
| ssl3044ζ | Ferredoxin-like protein | 10.3 ± 0.0 | 1.3 ± 0.1 | 6.5 ± 0.7 | 2.3 ± 0.0 | |
| slr0967ɛ' | Protein of unknown function | 9.8 ± 0.6 | 1.2 ± 0.1 | 8.4 ± 0.1 | 2.2 ± 0.2 | |
| slr2076γ | groEL1 | 60-kD chaperonin 1 | 8.7 ± 1.5 | 16.4 ± 2.4 | 3.7 ± 0.0 | 11.0 ± 1.8 |
| slr1915 | Protein of unknown function | 8.5 ± 0.8 | 1.2 ± 0.0 | 6.0 ± 0.3 | 3.4 ± 0.0 | |
| slr0589 | Protein of unknown function | 7.8 ± 0.5 | 1.8 ± 0.1 | 9.6 ± 0.7 | 1.4 ± 0.1 | |
| slr1686ζ' | Protein of unknown function | 7.6 ± 0.5 | 0.9 ± 0.0 | 3.5 ± 0.1 | 1.4 ± 0.1 | |
| ssl1633 | hliC | CAB/ELIP/HLIP superfamily | 6.9 ± 0.5 | 1.5 ± 0.1 | 3.6 ± 0.2 | 5.4 ± 1.1 |
| slr1597β' | Protein of unknown function | 6.7 ± 1.0 | 1.0 ± 0.0 | 4.1 ± 0.4 | 1.5 ± 0.0 | |
| sll1433 | Protein of unknown function | 6.1 ± 0.3 | 2.2 ± 0.3 | 6.5 ± 0.0 | 2.4 ± 0.1 | |
| slr1204 | htrA | Ser protease | 6.0 ± 0.7 | 2.5 ± 0.1 | 3.1 ± 0.6 | 3.0 ± 0.3 |