Table IV.

Results of analysis by 2D-DIGE of changes in levels of proteins upon mutation of the hik34 gene and heat shock in Synechocystis

The first column of numbers indicates ratios of levels of individual proteins in wild-type and ΔHik34 cells grown at 30°C. The second and third columns show changes in relative levels of proteins after heat shock due to incubation at 42°C for 60 min. This table lists six proteins that we identified in previous studies (Simon et al., 2002) and that we also identified as products of heat-inducible genes by DNA microarray analysis (Table III). The values are averages of results of five independent experiments, which gave a statistical confidence limit of 95%.

Gene Product	Protein	ΔHik34/Wild Type	Heat Shock Induction at 42°C, 60 min
		30°C	Wild Type (42°C/30°C)	ΔHik34 (42°C/30°C)
GroEL	60-kD chaperonin	2.5	5.3	3.8
DnaK2	HSP 70	2.8	2.5	1.8
HspA	Small HSP	1.6	19.9	21.0
ClpB	Subunit of Clp protease	1.8	2.6	3.6
HtpG	HSP 90	3.8	2.4	1.9
GroES	10-kD chaperonin	2.0	3.2	2.6