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Time course of LYCH distribution after injection into a SE of the stem phloem in V. faba tissue slices. A to C, Immediately after injection into the SE, the dye accumulated in the nuclei of the CCs (A), after 0.5 h the dye was evenly distributed over the SE/CC compartments (B), and after 17.5 h LYCH was nearly completely accumulated by the vacuole of the CCs (C). No LYCH traffic into the adjoining PPCs was visible. Arrows point to the nuclei of the CCs. Bar = 100 μm.
