Representative in vivo 1H-MAS NMR (A) and 13C-MAS NMR (B) spectra of tobacco seeds. A, The area below the H2O signal at 4.8 ppm was used to calculate the relative water uptake of tobacco seeds with time after the start of imbibition in medium without (Con) and with (ABA) 10 μm ABA, which is presented in Figure 2B. B, The assignment of glycerol and fatty acid peaks according to Bardet et al. (2001). Glycerol (d and e), C18:1Δ9 + C18:2 (a, b, i, j, and m–o), C18:2 (c, h, and l), C18:1Δ9 (f and g). The relative contents per seed presented in Figure 2C were calculated from the corresponding peak heights.