Figure 1.
Top, Diagram of AGPase small-subunit mosaics analyzed in these studies. Maize (white) is the top subunit, while potato (black) is listed at the bottom. The mosaics are listed in the middle. Numbers between arrows (13, 9, and 34) signify the number of differing amino acids in that region. The first mosaic, MP, contains maize amino acids from position 1 to 199, while the second mosaic, P2M2P, contains maize amino acids from position 73 to 199. Maize contains 25 N-terminal amino acids not present on the potato small subunit. The numbering convention is based on the maize enzyme. The potato enzyme does not include the localization signals on the N terminus. Mosaics were expressed in E. coli with the maize endosperm large subunit, Mls. Bottom, Alignment of the maize and potato small-subunit amino acids. Sequences were aligned using the 5.4.1 Multalin version (Corpet, 1988).