Table I.

Purification of maize endosperm AGPase expressed in E. coli^a

Purification Step	Volume	Protein	Total	Specific Activity	Fold Purification	Total Units	% Recovery
	mL	mg/mL	mg	μmol min−1 mg−1
Crude supernatant	20	12.71	254.2	0.75		191	100
Protamine sulfate	25	4.53	113.3	1.6	2	184	97
45% Ammonium sulfate	1.2	17.57	21.1	6.5	8.7	137	72
High Q	18	0.45	8.2	11.5	15.3	94	44
Concentrate	2.5	1.61	4.0	10.9	14.5	44	23
Hydroxyapetite	16	0.07	1.0	31.9	42.5	33	18
Concentrate	0.15	3.49	0.5	41.1	54.9	21	11

^aData correspond to a typical purification starting with approximately 250 mg of crude extract from E. coli. Assays were performed with assay A (reverse direction) using standard reaction conditions.