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Observed kinetic constants of wild-type AGPase and the small-subunit mosaics in the absence of 3-PGA
| Mutant
|
ATP
|
Glc-1-P
|
||||
|---|---|---|---|---|---|---|
| S0.5 | Vmax(obs) | kcat | S0.5 | Vmax(obs) | kcat | |
| mm | μmol min−1 mg−1 | s−1 | mm | μmol min−1 mg−1 | s−1 | |
| Maize AGPase | 0.84 | 1.4 | 5.1 | 1.3 | 1.6 | 5.9 |
| MPss/Mls | 0.44 | 7.9 | 30.0 | 0.4 | 8.8 | 32.3 |
| P2M2Pss/Mls | 0.60 | 3.1 | 11.4 | 0.9 | 3.5 | 12.8 |
| Pss/Mls | 0.82 | 1.3 | 4.8 | 0.9 | 1.4 | 5.1 |
