Figure 1.

Molecular masses of the subunits of a native β-galactosidase from radish seeds. A, Enzyme protein (0.5 μg) purified from imbibed radish seeds by several chromatographic procedures was separated on SDS-PAGE. Lane S, Molecular mass markers; lane 1, purified enzyme. Protein in the gel was stained with Coomassie Brilliant Blue R-250. The protein bands subjected to peptide sequence analysis are indicated by arrows. B, Molecular masses of the subunits were determined by MALDI-TOF/MS. The numbers at the top of the peaks indicate the observed molecular mass.