Figure 6.
Hydrolysis of mixed-linkage galactooligosaccharides. Hydrolysis products of β-Gal-(1→6)-β-Gal-(1→3)-Gal and β-Gal-(1→3)[β-Gal-(1→6)]-Gal by action of rRsBGAL1 were analyzed on TLC. Lane S, Standard Gal, β-(1→3)-galactobiose, and β-(1→6)-galactobiose; lane 1, β-Gal-(1→6)-β-Gal-(1→3)-Gal before hydrolysis; lane 2, hydrolysis products of β-Gal-(1→6)-β-Gal-(1→3)-Gal after 1 h; lane 3, those after 12 h; lane 4, β-Gal-(1→3)[β-Gal-(1→6)]-Gal before hydrolysis; lane 5, hydrolysis products of β-Gal-(1→3)[β-Gal-(1→6)]-Gal after 1 h; lane 6, those after 12 h. Localization of the standard sugars is indicated on the left side.