Table II.

Substrate specificity of rRsBGAL1 toward oligosaccharides

Substratea	Relative Activityb
β-(1→3)-Galactooligosaccharides
β-(1→3)-Galactobiose	32
β-(1→3)-Galactotriose	44
β-(1→4)-Galactooligosaccharides
β-(1→4)-Galactobiose	0
β-(1→4)-Galactotriose	0
β-(1→4)-Galactotetraose	0
β-(1→6)-Galactooligosaccharides
β-(1→6)-Galactobiose	27
β-(1→6)-Galactotriose	49
β-(1→6)-Galactotetraose	68
β-(1→6)-Galactopentaose	50
Heterooligosaccharides
β-Gal-(1→6)-β-Gal-(1→3)-Gal	40
β-Gal-(1→3)[β-Gal-(1→6)]-Gal	42
β-Gal-(1→3)-Arap	21
β-Gal-(1→3)-GalNAc	0.5
β-Gal-(1→3)-GlcNAc	0.5
β-Gal-(1→4)-Glc (lactose)	11
β-Gal-(1→4)-Man	0.4
α-l-Ara-(1→3)-β-Gal-(1→6)-Gal	0
β-GlcUA-(1→6)-Gal	0
β-GlcUA-(1→6)-β-Gal-(1→6)-Gal	0
4-Me-β-GlcUA-(1→6)-Gal	0
4-Me-β-GlcUA-(1→6)-β-Gal-(1→6)-Gal	0
4-Me-β-GlcUA-(1→6)- β-Gal-(1→6)- β-Gal-(1→3)-Gal	0
Others
PNP-β-Gal	100
Methyl-β-galactoside	4
Laminaribiose [β-Glc-(1→3)-Glc]	0
Cellobiose [β-Glc-(1→4)-Glc]	0
Gentiobiose [β-Glc-(1→6)-Glc]	0

^aThe enzyme was incubated with substrates at a concentration of 5 mm, and 2 mm was employed for PNP-β-Gal.

^bActivity is expressed as percent of that (64.9 units mg protein⁻¹) of PNP-β-Gal.