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. 2005 Jul;138(3):1563–1576. doi: 10.1104/pp.105.062562

Table III.

Kinetic values of rRsBGAL1

Substratea Km kcat kcat/Km
mm s−1 m−1s−1
PNP-β-Gal 0.30 123.0 4.10 × 105
β-(1→3)-Galactobiose 1.77 28.6 0.16 × 105
β-(1→6)-Galactobiose 0.77 32.5 0.42 × 105
a

To examine the effects of galactooligosaccharides on the activity, reactions were carried out with varying concentrations of PNP-β-Gal (0.1–5 mm), β-(1→3)-galactobiose (0.5–10 mm), and β-(1→6)-galactobiose (0.5–10 mm). The Km and kcat values were calculated from a Hanes-Woolf plot using the obtained activities.