Table I.
Effects of microtubule-depolymerizing compounds on blue light-dependent stomatal opening in the Vicia leaf epidermis
Vicia epidermal peels were treated with microtubule-depolymerizing compounds and illuminated with red light at 150 μmol m−2 s−1 or blue light at 15 μmol m−2 s−1 under the red light background at150 μmol m−2 s−1. A total of 10 μm trifluralin, 10 μm oryzalin, and 20 μm propyzamide were used in the experiment. All these compounds were solubilized in dimethyl sulfoxide (DMSO) and applied at the final concentration of 0.1% DMSO. As a control, the measurement was done at a final concentration of 0.1% DMSO. Blue light-specific opening was given by the following equation: Blue light-specific opening = Blue light-dependent opening minus red light-dependent opening.
| Stomatal Aperture (μm) ± se (n = 40)
|
||||
|---|---|---|---|---|
| Dark | Red Light | Red + Blue Light | Blue Light-Specific Opening | |
| Control | 3.43 ± 1.06 | 6.47 ± 1.34 | 10.19 ± 0.70 | 3.72 (100%) |
| Trifluralin | 6.37 ± 1.47 | 8.20 ± 1.10 | 1.83 (49.2%) | |
| Control | 3.79 ± 1.03 | 5.66 ± 1.11 | 9.54 ± 0.79 | 3.88 (100%) |
| Oryzalin | 5.89 ± 1.01 | 7.40 ± 1.16 | 1.51 (38.9%) | |
| Control | 3.43 ± 1.06 | 6.47 ± 1.34 | 10.19 ± 0.70 | 3.72 (100%) |
| Propyzamide | 6.26 ± 1.40 | 9.22 ± 1.11 | 2.96 (79.6%) | |