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Amino acid sequence of α-l-arafase (PpARF 2) from Japanese pear fruit. The shade-boxed amino acids are the sequences of N-terminal amino acids and tryptic peptide fragments generated from purified α-l-arafase. White-boxed amino acid sequences exhibited possible N-glycosylation sites. The cleaved signal peptide is indicated by an underline. The putative catalytic nucleophile (amino acid position 292) and catalytic acid/base (amino acid position 498) are circled.
