Table I.
Potato tuber proteins identified from cultivar Desirée
The assigned spots are shown in Figure 1. The accession number/EST refers to database accession numbers of matching proteins or ESTs; the full peptide sequences are shown where the ESTs are not publicly available. The number of matched peptides and the functional group are also indicated. The first group includes proteins with statistically significant differences between GM lines or controls; the second group includes proteins that showed significant differences between some of the non-GM genotypes and had high loadings in the second, third, or fourth principal component; the third group includes other identified proteins. Where two or more proteins were identified in the same spot, all are listed.
| Spot | Identification | Accession No./EST | Peptides | Functional Group |
|---|---|---|---|---|
| Proteins with Significant Differences between the Non-GM Varieties and Landraces | ||||
| Second Component | ||||
| 4 | Thioredoxin peroxidase/peroxiredoxin | AF442385, AAP34571 | 6 | Disease/defense |
| 55 | RAN1, GTP binding nuclear protein | NP_197501, T15nr049 | 1 | Intracellular traffic |
| 56 | Cathepsin D inhibitor | AAG12337 | 1 | Disease/defense |
| 57 | Putative Gln amidotransferase/cyclase | BAC43102 | 1 | Metabolism |
| 58 | Expressed protein | NP_179517 | 2 | Unclassified |
| 59 | Patatin; Dehydrin homolog C17 | P15478, PSTFS38TH, PSEAF25TH; T07779 | 9; 3 | Protein destination and storage; disease/defense |
| 60 | EST (peptide sequences LGSHFVSENQDVSIK VAYSIVGPTHSPLR FSTSSSSTK YETGRPHSYK YETGRPHSYKLR IEKYETGRPHSYKLR) | T04nr049 | 6 | Unclassified |
| Third Component | ||||
| 6 | Triosephosphate isomerase, cytosolic | P12863, P48494 | 4 | Energy, metabolism |
| 14 | Enolase | P26300, AAL06912 | 9 | Energy |
| 15 | UTP-Glc-1-P uridylyltransferase | P19595, AAB71613 | 13 | Metabolism |
| 20 | Enolase | P26300, Q42971 | 13 | Energy |
| 43 | Enolase | JQ1186, P26300 | 8 | Energy |
| 44 | Enolase | P26300, JQ1186 | 10 | Energy |
| 45 | UTP-Glc-1-P uridylyltransferase | P19595 | 16 | Metabolism |
| 61 | 26S protease regulatory subunit 6A homolog (TBP-1) | P54776 | 12 | Protein destination and storage |
| 62 | Fru-bisP aldolase, cytoplasmic isozyme 2; Patatin; Phosphoenolpyruvate carboxylase kinase | P46257; P15478; AAF19403 | 1; 2; 1 | Energy; protein destination and storage; signal transduction |
| 63 | Ascorbate peroxidase | BAC22953, CAB58361, NP_195321 | 4 | Disease/defense |
| 64 | Enolase | JQ1186, P26300 | 8 | Energy |
| 65 | Asp-semialdehyde dehydrogenase precursor; Putative protein disulfide isomerase | AAG33078; BAB67990 | 1; 5 | Metabolism; protein destination and storage |
| 66 | Enolase | JQ1186, P26300 | 5 | Energy |
| 67 | Ascorbate peroxidase | CAB58361, BAC22953, NP_195321, PSHBN28TH | 6 | Disease/defense |
| Fourth Component | ||||
| 47 | Phytepsin precursor (aspartic proteinase) | P42210, PSTDE14TH | 4 | Protein destination and storage |
| 68 | Expressed protein/hypothetical protein/unknown protein | NP_568098, T02532, AAO18441, PSHBQ20TH | 3 | Unclassified |
| 69 | Patatin | P15478 | 3 | Protein destination and storage |
| 70 | Kunitz-type enzyme inhibitor S0C11 | AAL67830, T04nr016 | Disease/defense | |
| 71 | EST | PSTGU28TH | 2 | Unclassified |
| 72 | Aspartic proteinase | AAC49730, PSTES05TH, PSHEN78TH | 5 | Protein destination and storage |
| Proteins with Significant Differences between the GM Lines | ||||
| 54 | Aspartic protease | AAT77954 | 3 | Protein destination and storage |
| 70 | Kunitz-type enzyme inhibitor S0C11 | AAL67830, T04nr016 | 2 | Disease/defense |
| 73 | Patatin | T07592 | 2 | Protein destination and storage |
| 74 | Hsp20.1 protein | CAA12387, 4H3p01nr | 8 | Disease/defense |
| 76 | Chaperonin 21 precursor | AAF6029 | 7 | Protein destination and storage |
| 77 | EST (peptide sequences LAIGTGNTFNLVFIK YLDTNGEAGHVK NVASWFQIK KTGSYMYK) | T02nr073 | 4 | Unclassified |
| 78 | Unknown protein | NP_908355 | 2 | Unknown |
| 75, 79 | Unidentified | |||
| Other Identified Proteins | ||||
| 1 | Glc-3-P dehydrogenase, cytosolic | P26519, S72667 | 2 | Energy |
| 2 | Pathogenesis-related protein STH-2 | P17642 | 1 | Disease/defense |
| 3 | Kunitz-type enzyme inhibitor S0C11 | AAL67830, AF460237 | 4 | Disease/defense |
| 5 | Superoxide dismutase [Cu-Zn] | P27082, P14830 | 2 | Disease/defense |
| 7 | Malate dehydrogenase, cytosolic; Glc-3-P dehydrogenase | CAC10208; AAB54003 | 1; 1 | Energy |
| 8 | Dihydrolipoamide dehydrogenase precursor | AF295339 | 1 | Energy |
| 9 | Putative malate dehydrogenase | NP_171936 | 1 | Energy |
| 10 | Proteasome subunit α-type 6 | Q9XG77 | 2 | Protein destination and storage |
| 11 | Transcription factor homolog BTF3 | T16984 | 1 | Transcription |
| 12 | Nucleoside-diphosphate kinase | S47974 | 1 | Metabolism |
| 13 | Heat shock protein 17.6 | T07602 | 1 | Disease/defense |
| 16 | UTP-Glc-1-P uridylyltransferase | AAB71613 | 1 | Metabolism |
| 17 | Kunitz-type enzyme inhibitor S9C11 | AAL67830 | 1 | Disease/defense |
| 18 | Triosephosphate isomerase precursor, chloroplast | P48496, Q9M4S8 | 3 | Energy, metabolism |
| 19 | Putative Ala aminotransferase | AAM61453 | 1 | Metabolism |
| 21 | β-Cyano-Ala synthase-like protein | BAB20032 | 1 | Metabolism |
| 22 | 26S proteasome, non-ATPase regulatory subunit (RPN11), putative | NP_197745 | 1 | Protein destination and storage |
| 23 | Cystatin | AAF23126 | 2 | Disease/defense |
| 24 | Phytepsin precursor (aspartic proteinase) | P42210 | 1 | Protein destination and storage |
| 25 | Probable malate dehydrogenase | T06386 | 1 | Energy |
| 26 | Patatin | P15478 | 1 | Protein destination and storage |
| 27 | Patatin; Phosphoenolpyruvate carboxykinase | P15478; AAF19403 | 1; 1 | Protein destination and storage; signal transduction |
| 28 | Patatin; Phosphoenolpyruvate carboxykinase | P15478; AAF19403 | 3; 1 | Protein destination and storage; signal transduction |
| 29 | Patatin | AAK56395 | 3 | Protein destination and storage |
| 30 | Fructokinase | P37829 | 4 | Metabolism |
| 31 | Fructokinase | AAB51108 | 3 | Metabolism |
| 32 | Putative lactoylglutathione lyase | AAL07227 | 1 | Disease/defense |
| 33 | Phytepsin precursor (aspartic proteinase) | P42210 | 1 | Protein destination and storage |
| 34 | Cys proteinase precursor | S55923 | 1 | Protein destination and storage |
| 35 | Heat shock protein 17.6 | T07602 | 1 | Disease/defense |
| 36 | ATP synthase β-chain precursor, mitochondrial | Q01859, AAD03393 | 7 | Unclear classification |
| 37 | UTP-Glc-1-P uridylyltransferase | S31431 | 4 | Metabolism |
| 38 | Actin | P30172, Q96493 | 3 | Cell structure |
| 39 | Putative nascent polypeptide-associated complex α-chain/expressed protein | BAB89723, NP_196889 | 3 | Protein destination and storage |
| 40 | Putative nascent polypeptide-associated complex α-chain/expressed protein | BAB89723, NP_196889 | 2 | Protein destination and storage |
| 41 | Ascorbate peroxidase | CAA72247, CAB58361 | 2 | Disease/defense |
| 42 | Ascorbate peroxidase | CAA72247, CAB58361 | 2 | Disease/defense |
| 46 | Annexin p34 | AAC97494 | 2 | Disease/defense |
| 48 | dnaK-type molecular chaperone hsc-2/heat shock protein 70 kD | S14950, CAB72129, PSHDH15TH | 4 | Protein destination and storage |
| 49 | Aminopeptidase 2 precursor, chloroplast | Q42876, PSEAS06TH, PSHCI03TH | 2 | Protein destination and storage |
| 50 | Patatin | AAK56395 | 1 | Protein destination and storage |
| 51 | Putative NAD-dependent malate dehydrogenase, mitochondrial | CAD33240 | 2 | Energy |
| 52 | Gly-rich RNA-binding protein GRP1 | CAA73034 | 1 | Transcription |
| 53 | Dihydrolipoamide dehydrogenase precursor | AAN23154 | 2 | Metabolism |