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. 2005 Jul 7;24(14):2700–2713. doi: 10.1038/sj.emboj.7600721

Figure 2.

Figure 2

RNAi-mediated knockdown of Drob-1 enhances polyglutamine-induced neuronal toxicity. (A, B) Expression of the UAS-drob-1-IR or UAS-MJDtr-Q78 transgene was targeted to the nervous system using the elav-GAL4 driver. We used two control fly lines: one bears only the elav-GAL4 transgene and the other bears both the UAS-lacZ-IR and elav-GAL4 transgenes. The elav>drob-IR, MJDtr-Q78(M) flies died earlier than the elav>MJDtr-Q78(M) flies. (C–J) Light micrographs of the eyes of flies 1 day (C, G), 10 days (D, H), 20 days (E, I), and 30 days (F, J) after eclosion are shown. (K–R) Eyes (K–N) and tangential sections of the eyes (O–R) of flies 1 day after eclosion are shown. (K, O) Control flies bearing only the driver GMR-GAL4 show normal eyes. Genotypes are as follows: GMR-GAL4/CySM1; UAS-MJDtr-Q78(M)/+ (C–F), GMR-GAL4/UAS-drob-1-IR; UAS-MJDtr-Q78(M)/+ (G–J), GMR-GAL4/CySM1 (K, O), GMR-GAL4/CySM1; UAS-MJDtr-Q78(M)/+ (L, P), GMR-GAL4/UAS-drob-1-IR; UAS-MJDtr-Q78(M)/+ (M, Q), and GMR-GAL4/UAS-drob-1-IR; TM3, Sb/+ (N, R).