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. 2005 Jul 7;24(14):2700–2713. doi: 10.1038/sj.emboj.7600721

Figure 8.

Figure 8

Knockdown of Drob-1 enhances rotenone- or 3-NP-induced mitochondrial morphological abnormalities and decreases activities of respiratory chain complexes in S2 cells. (A–F) Electron microscopy images of mitochondria in EGFP dsRNA- or drob-1 dsRNA-treated S2 cells with or without the treatment with rotenone or 3-NP (arrowheads: mitochondria). Rotenone or 3-NP induced swollen and lower electron-dense mitochondria with disrupted outer membranes, signs of degeneration and derangement of the membranes (arrows). Scale bars correspond to 500 nm. (G) Quantification of mitochondrial morphological abnormalities. Mitochondria were classified into three types based on their morphologies: normal mitochondria, swollen and higher electron-dense mitochondria, and swollen and lower electron-dense mitochondria with abnormal membrane structures. Approximately 150 mitochondria in at least 15 cells were analyzed in an experimental group. (H, I) Mitochondrial complex I+III and complex II+III activities. Mean±s.e.m., n=3, *P<0.05, **P<0.005, and ***P<0.0005 for cells with drob-1 dsRNA compared with cells with EGFP dsRNA, †P<0.05, ††P<0.005, and †††P<0.0005 for cells with rotenone or 3-NP as compared with cells without drugs (none) by paired Student's t-test.