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. 2005 Jun 23;24(14):2491–2498. doi: 10.1038/sj.emboj.7600732

Figure 5.

Figure 5

Blocking of fluorescently labelled CFP-10·ESAT-6 complex binding to U937 cells by competition with unlabelled complex. (A) Typical fluorescence images obtained for U937 cells after incubating with 1 μM Alexa Fluor 546-labelled CFP-10·ESAT-6 complex for 15 min at 4°C. (B) The dramatic effect of adding a 20-fold molar excess of unlabelled complex. Clearly, the fluorescence labelling of U937 cells in the presence of an excess of unlabelled CFP-10·ESAT-6 complex is several orders of magnitude lower, which indicates that binding is mediated via the protein complex and not the attached fluorophore. The size bars shown correspond to 10 μm.