Figure 5.
The C-terminal domain of BLM is required for efficient unwinding of DHJs. Helicase assays using DHJ/Rsa1 and a series of two-fold dilutions of BLM, BLM(213–1417) or BLM(213–1267) on DHJ/Rsa1, as indicated. The positions of DHJ/Rsa1 and the unwound product are shown on the right. The graph shows quantification of the level of unwound product generated by BLM (circles), BLM(213–1417) (squares) and BLM(213–1267) (triangles) on DHJ/Rsa1. Note that the scale on the horizontal axis is logarithmic.