Skip to main content
. 2005 Jun 30;24(14):2590–2601. doi: 10.1038/sj.emboj.7600742

Figure 2.

Figure 2

Serum-induced expression of ATF3 via ERK/MAPK signaling pathway. (A) Effect of MAPK inhibitors on serum-induced ATF3 expression in wild-type TGR1 cells determined by Western blot. The inhibitors were added 2 h before adding serum, and cell extracts were prepared 12 h after serum treatment. SP600125 and SB203580 were used at 10 and 25 μM, respectively. DMSO indicates vehicle only. (B) Phosphorylated ERK (P-ERK) and ERK (ERK) were measured at the indicated time after serum treatment of wild-type and c-myc-deficient cells by Western blot. Both 42 and 44 kDa isoforms were detected. In the lower panel, the activity 10 min after serum treatment in the presence of 25 μM PD98059 is shown.

HHS Vulnerability Disclosure