Table 1.
Cell cycle parameters of wild, c-myc−/−, and A2 cells
c-myc+/+ |
−/− |
A2 |
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G1 | S | G2/M | G1 | S | G2/M | G1 | S | G2/M | |||||||||||||||||||||||||||||||||||||||||
Cell cycle phase (h)a | 9.2 | 4.8 | 4.6 | 29.9 | 6.6 | 10.3 | 11.8 | 6.9 | 8.1 | ||||||||||||||||||||||||||||||||||||||||
Total doubling time (h)b | 18.6 | 46.8 | 26.8 | ||||||||||||||||||||||||||||||||||||||||||||||
Cell cycle distribution (%)c | 33.4 | 58.6 | 8 | 56.7 | 28.4 | 14.9 | 37.3 | 39.2 | 23.5 | ||||||||||||||||||||||||||||||||||||||||
Cell cycle phase (h)d | 6.2 | 10.9 | 1.5 | 26.5 | 13.3 | 7.0 | 10.0 | 10.5 | 6.3 | ||||||||||||||||||||||||||||||||||||||||
Mitotic shake-off (h)e |
4.6 |
|
|
30.4 |
|
|
11.7 |
|
|
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aCell cycle phase was calculated from cell cycle distributions based on flow cytometric analysis. | bDoubling time was calculated from cell cycle distributions based on growth rates. | bCell cycle distributions shown in Figure 7A were corrected using BrdU incorporation values of 58.6% (c-myc+/+ cells), 28.4% (c-myc−/− cells), and 39.2% (A2 cells), respectively. These values were determined by pulse labeling of BrdU as in Materials and methods. | dCorrected cell cycle distributions were combined with growth rate values (doubling time). | eG1 phase duration from data in Figure 7B. Values for 50% labeling index are shown. |