Figure 2.

Detection and analysis of autophagosomes and lysosomes. (A) Detection of intracellular autophagic vesicles by fluorescence microscopy; (B) analysis of the average fluorescence value of Lyso-Tracker Red; (C) fluorescent probe localization of mitochondria (green) and lysosomes (red) in living cells; (D) analysis of the average fluorescence values of Lyso-Tracker Red. The data are expressed as the mean ± SD (n= 3) of three experiments. The statistical significance of differences between groups was analyzed using one-way ANOVA, the same letters indicate the difference is not significant, while different letters indicate the difference is significant (p < 0.05).