Figure 3.
Detection and analysis of mTOR, p-mTOR, AMPK, p-AMPK, LC3B, and p62 expression by Western blot. (A) WB results of mTOR, p-mTOR, AMPK, p-AMPK, LC3B, and p62; (B) grayscale analysis of p-mTOR/mTOR; (C) grayscale analysis of p-AMPK/AMPK; (D) grayscale analysis of LC3B-II/LC3B-I; (E) grayscale analysis of p62. The data are expressed as the mean ± SD (n = 3) of three experiments. The statistical significance of differences between groups was analyzed using one-way ANOVA, the same letters indicate the difference is not significant, while different letters indicate the difference is significant (p < 0.05) (Supplementary File S1).
