Figure 2.

Transcriptional diversity of CD4⁺ and CD8⁺ T cells. (A) Uniform Manifold Approximation and Projection (UMAP) of 13 subclusters identified in CD4⁺ and CD8⁺ T cells. (B) Dot plots showing distinct expressions of the selected marker genes in each subcluster. (C) Heatmap illustrating the scaled score calculated based on the expression of curated gene signatures across CD4⁺ T cell subclusters (left) and CD8⁺ T cell subclusters (right). (D) Heatmap of the top 10 differentially expressed genes (ranked by log-transformed fold change in descending order) in the CD4⁺ and CD8⁺ T cell subclusters. (E) Box plots showing the percentage of cells for each T cell subcluster in the keloid and normal scar samples. The p-value indicated in the plot was calculated by unpaired two-tailed t-tests. (F) The proportion of each T cell subcluster in three keloid samples and three normal scar samples. K, keloid; N, normal scar. (G) Violin plots showing differentially expressed genes in the Th17 cells in keloids and normal scars. K, keloid; N, normal scar. (H) Functional KEGG pathway enrichment of the upregulated genes (keloid vs. normal scar, avg_logFC > 0.25, and p value < 0.05) in Th17 cells. The p-value was calculated using the hypergeometric distribution and corrected using the Benjamini and Hochberg method. Pathways with an adjusted p-value of <0.05 are considered significant.