Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1971 Oct;124(5):915–919. doi: 10.1042/bj1240915

The preparation of an alkali-soluble collagen from demineralized bone

G D Kemp 1,*, G R Tristram 1
PMCID: PMC1177279  PMID: 4943353

Abstract

Ossein was solubilized by the action of alkali and a resulting high-molecular-weight fraction isolated. The chemical and physical properties of this fraction were studied and compared with those of an acid-soluble collagen prepared from calf skin by conventional techniques. From the results it is concluded that the alkali-soluble protein exhibits only minor differences from acid-soluble collagen, and that these differences can be ascribed for the most part to a decrease in the inter- and intramolecular cross-linking.

Full text

PDF
915

Images in this article

916-1PLATE 1
on p.916-1

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALTGELT K., HODGE A. J., SCHMITT F. O. Gamma tropocollagen: a reversibly denaturable collagen macromolecule. Proc Natl Acad Sci U S A. 1961 Dec 15;47:1914–1924. doi: 10.1073/pnas.47.12.1914. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BOWES J. H., KENTEN R. H. The swelling of collagen in alkaline solutions; swelling in solutions of univalent bases. Biochem J. 1950 May;46(5):524–529. doi: 10.1042/bj0460524. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BOWES J. H. The swelling of collagen in alkaline solutions; swelling in solutions of bivalent bases. Biochem J. 1950 May;46(5):530–532. doi: 10.1042/bj0460530. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bornstein P., Piez K. A. The nature of the intramolecular cross-links in collagen. The separation and characterization of peptides from the cross-link region of rat skin collagen. Biochemistry. 1966 Nov;5(11):3460–3473. doi: 10.1021/bi00875a012. [DOI] [PubMed] [Google Scholar]
  5. Bowes J. H., Kenten R. H. The amino-acid composition and titration curve of collagen. Biochem J. 1948;43(3):358–365. doi: 10.1042/bj0430358. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bowes J. H., Kenten R. H. The swelling of collagen in alkaline solutions. 1. Swelling in solutions of sodium hydroxide. Biochem J. 1950 Jan;46(1):1–8. doi: 10.1042/bj0460001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. CLARKE J. T. SIMPLIFIED "DISC" (POLYACRYLAMIDE GEL) ELECTROPHORESIS. Ann N Y Acad Sci. 1964 Dec 28;121:428–436. doi: 10.1111/j.1749-6632.1964.tb14214.x. [DOI] [PubMed] [Google Scholar]
  8. COURTS A. Structural changes in collagen. The action of alkalis and acids in the conversion of collagen into eucollagen. Biochem J. 1960 Feb;74:238–247. doi: 10.1042/bj0740238. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Chibnall A. C., Rees M. W., Williams E. F. The total nitrogen content of egg albumin and other proteins. Biochem J. 1943 Sep;37(3):354–359. doi: 10.1042/bj0370354. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Chwabe C. Disc electrophoresis (destaining, staining, protein recovery). Anal Biochem. 1966 Nov;17(2):201–209. doi: 10.1016/0003-2697(66)90198-9. [DOI] [PubMed] [Google Scholar]
  11. Clark C. C., Veis A. A high-resolution acrylamide-gel electrophoresis technique for the analysis of collagen polymer components. Biochim Biophys Acta. 1968 Jan 22;154(1):175–182. doi: 10.1016/0005-2795(68)90269-9. [DOI] [PubMed] [Google Scholar]
  12. KUEHN K., ZIMMER E., WAYKOLE P., FIETZEK P. DIE WIRKUNG VON ALKALI AUF KOLLAGEN. DIE ANDERUNG DES LADUNGSMUSTERS DER TROPOKOLLAGEN-MOLEKUELE SOWIE DIE SPALTUNG DER INTRA- UND INTERMOLEKULAREN BINDUNGEN. Hoppe Seylers Z Physiol Chem. 1963;333:209–217. doi: 10.1515/bchm2.1963.333.1.209. [DOI] [PubMed] [Google Scholar]
  13. STEVEN F. S., TRISTRAM G. R. The presence of non-protein nitrogen in acetic acid-soluble calf-skin collagen. Biochem J. 1962 May;83:240–244. doi: 10.1042/bj0830240. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES