Fig. 1.

Primary sequence representation of the mouse and human Keap1 proteins and the most readily modified cysteines. (A) The cysteines in mouse Keap1 most readily modified by dexamethasone 21-mesylate (12) are shown in green. All cysteines shown were modified by BIA in this study, both green- and red-labeled, and those in red were found uniquely in this study. Those labeled with an asterisk were reported to be important in the regulation of Nrf2 ubiquitination (5). (B) The order of reactivity of the cysteines determined in this study is shown, along with the ratio of BIA to Keap1 required to obtain those modifications.