Fig. 1.

NCS-1 immunoreactivity is present in pancreatic β cells. (A) Representative image of a section of rat pancreatic islet immunostained with antisera to NCS-1 (Left) and insulin (Center) and their overlay (Right). (Scale bar: 50 μm.) (B) Western blot showing presence of NCS-1 protein in NMRI mouse islets and INS-1E cells. (C) Subcellular localization of NCS-1 in INS-1E cells. Subcellular fractions were obtained by sucrose density gradient centrifugation. NCS-1 and organelle markers were detected by using specific antibodies. The experiment was repeated three times with similar results. GK, glucokinase; PM, plasma membrane; ER, endoplasmic reticulum; CGA, chromogranin A; SG, secretory granule.