Fig. 1.

Characterization of the p195–212-specific T cell line. (A) SJL mice were immunized with p195–212 (10 μg per mouse in complete Freund's adjuvant), and LN cells were harvested 10 days later. p195–212-specific T cell line was established and maintained as described in Materials and Methods. Cells were stained with PE-coupled anti-CD4 specific antibody 7 days after antigenic stimulation and analyzed by FACS. (B) Cells of the line (n = 10⁴ per well) were stimulated with various concentrations of p195–212 in the presence of irradiated syngeneic splenocytes (n = 0.5 × 10⁶ per well). (C) Cells were incubated with p195–212 (1 μg per well) in the presence or absence of various doses of the dual APL or the reversed dual APL. (B and C) The proliferation assay was performed as described in Materials and Methods. Results are expressed as mean cpm of triplicates ± SD values, and they represent one experiment of four performed.