The effect of the dual APL on cytokine secretion by the p195–212-specific T cells. Cells of the line (n = 106 per ml) were stimulated with p195–212 (5 μg per ml) with or without the dual APL or the reversed dual APL (500 μg per ml) for 24–48 h. Supernatants were collected, and concentrations of IFN-γ (A), IL-2 (B), TGF-β (C), and IL-10 (D) were determined by ELISA. Results are mean concentrations of triplicates ± SD values. *, P < 0.005; **, P < 0.0001; as compared with supernatants of cells stimulated with p195–212 alone.