Fig. 5.

Foxp3 and neuropilin are up-regulated in p195–212-specific T cells by the dual APL. T cells (n = 10⁶ per ml) of the line were stimulated with p195–212 (5 μg per ml) with or without the dual or the reversed dual APL (500 μg per ml) for 24 h. (A) Total RNA was extracted, and real-time PCR was performed for Foxp3 expression as described in Materials and Methods. Results are expressed as the mean percentage of the gene expression of triplicates ± SD values. ^*, P < 0.0005 when gene expression in the presence of the dual APL was compared with that in the absence of the dual APL. (B) Whole-cell lysates were prepared, separated on SDS/PAGE, and transferred to a nitrocellulose membrane that was later blotted with anti-neuropilin or anti-β-actin. Results are expressed as densitometry units (measured with National Institutes of Health image software), and they represent one of two experiments performed.