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. 2025 Jan 29;53(3):gkae1325. doi: 10.1093/nar/gkae1325

Figure 2.

Figure 2.

Disruptions of Mitochondrial Ribosome Assembly in Wbscr16 Knockout Adipose Tissues. (A and B) Representative western blots of mtDNA-encoded proteins (MT-ND4, MT-ND6, MT-CO1, MT-CO2 and MT-ATP6) (A), as well as nuclear genome-encoded mitochondrial proteins (NDUFA4, NDUFB8, UQCRC2, SDHA, SDHB, COX4, ATP5A and VDAC1) (B) in iBAT were shown (n = 4). (C) qRT-PCR analysis of Wbscr16 and two mtDNA-encoded rRNAs in iBAT from controls and AWBSCR16 KO mice was shown (n = 4). (D) qRT-PCR analysis of Wbscr16 and mtDNA encoded mRNAs in iBAT from floxed and AWBSCR16 KO mice was shown (n = 4). (E) Representative western blots of WBSCR16, the mtLSU protein MRPL37 and MRPL12, and the mtSSU protein MRPS35 and MRPS16 in iBAT from controls and AWBSCR16 KO mice were shown (n = 4). (F) The distribution of WBSCR16, the mtLSU protein MRPL12, and the mtLSSU protein MRPS35 and MRPS16 in mitochondrial ribosome fractions of iBAT from controls and AWBSCR16 KO mice were shown (n = 4). (G–I) The distribution of the mtLSU protein MRPL12 (G), the mtLSSU protein MRPS35 (H) and MRPS16 (I) in mitochondrial ribosome fractions of iBAT from controls and AWBSCR16 KO mice was quantified using ImageJ software. Data were presented as mean ± SEM; t-test: *P < 0.05, **P < 0.01, ***P < 0.001; NS, not significant.