Skip to main content
. 2025 Jan 29;53(3):gkae1325. doi: 10.1093/nar/gkae1325

Figure 4.

Figure 4.

Disrupted Binding of WBSCR16 to 16S rRNA in the absence of RBD. (A) RIP of FLAG-tagged WBSCR16 in MEFs was performed using FLAG magnetic beads or IgG magnetic beads, followed by qRT-PCR analysis of the eluted rRNA and mRNA (n = 6). (B) Protein structure of human WBSCR16 (PDB ID: 5XGS) illustrated regions encoded by different exons (pink; Exon 1–8 and 10–11, red; Exon 9). (C) Schematic illustrations of the exons in wild-type Wbscr16 (WT) and its ninth exon-deleted mutant (Del) were shown. (D) Representative western blots of FLAG tag and WBSCR16 in wild-type WBSCR16 (WT) or its ninth exon-deleted mutant (Del) after overexpressing them into MEFs were shown. (E and F) RIP of FLAG-tagged wild-type WBSCR16 (WT) and its ninth exon-deleted mutant (Del) from MEFs by FLAG magnetic beads or IgG magnetic beads. qRT-PCR analysis of eluted 16S rRNA (E) and 12S rRNA (F) was shown. Data were normalized to Gapdh (n = 3). Data were presented as mean ± SEM; t-test: *P < 0.05, ***P < 0.001; NS, not significant.