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. 2025 Jan 29;53(3):gkae1325. doi: 10.1093/nar/gkae1325

Figure 6.

Figure 6.

Increased fatty acid utilization in Wbscr16 knockout adipose tissues to prevent obesity. (A and B) Levels of fed and fasted glucose (A) and plasma NEFA (B) in floxed and AWBSCR16 KO mice were shown (n = 7–10). (C) A Heatmap displaying mRNA levels of upregulated or downregulated genes in iBAT from floxed and AWBSCR16 KO mice was shown (n = 3). (D) Glucose utilization during 24 and 48 h in mature adipocytes derived from floxed iBAT-SVF transduced with or without Cre lentivirus was shown. Values were normalized by total protein (n = 3–4). (E and F) Representative staining (E) and quantitative analysis (F) of the BODIPY C12 accumulated in LDs of mature adipocytes derived from iBAT-SVF transduced with or without Cre lentivirus were shown (n = 4–5). (G) Quantitative analysis of palmitic acid utilization during 24 h in mature adipocytes derived from iBAT-SVF infected with or without Cre lentivirus was shown (n = 3). (H and I) Representative PET-CT images (H) in coronal, sagittal and transaxial views and the ratio of SUV Max (I) (n = 6) of iBAT (arrows) from floxed and AWBSCR16 KO mice were shown. (J) Representative western blots of CD36, p-HSLSer660, total-HSL and CPT1α in iBAT from floxed and AWBSCR16 KO mice were shown. (K and L) Growth curves (K) (n = 6, two-way analysis of variance) and representative images (L) of HFD-treated floxed and AWBSCR16 KO mice were shown. (M–O) Representative images and HE staining of iBAT (M), iWAT (N) and eWAT (O) from mice after HFD treatment were shown. (P) Representative images, HE staining, and oil red O staining of the liver from HFD-treated mice were shown. (Q) Relative tissue weights normalized to body weight in chow diet (CD) or HFD fed Floxed and AWBSCR16 KO mice were shown (n = 4–11). (R and S) Levels of fed and fasted glucose (R), and fasted plasma NEFA (S) in HFD-treated floxed and AWBSCR16 KO mice were shown (n = 4–5). (T) Representative western blots of WBSCR16, MT-CO2 and PPARγ in iBAT from CD or HFD mice were shown. (U) Analysis of human WBSCR16 mRNA levels in adipose tissues from obese and non-obese subjects (BMI 16.7–50.2) with normal or impaired glucose tolerance (GSE27951) was shown (n = 11–13). Data were presented as mean ± SEM; t-test: *P < 0.05; **P < 0.01; ***P < 0.001.