Figure 2.

Structure of attenuated Src kinase domain/Cas chimeras. The Src/Cas(SD) chimeras were constructed as described in the materials and methods. Src* is the isolated attenuated Src kinase domain (Y416F). Src*/Cas(SD) is a fusion of the attenuated Src kinase domain to a.a. L¹⁵⁷ to R⁵¹⁶ of the Cas substrate domain. Src*/Cas(5'SD) is a fusion of the attenuated Src kinase domain to the 5' portion of the Cas substrate domain (a.a. L¹⁵⁷ to A³²⁴). Src*/Cas(3'SD) is a fusion of the attenuated Src kinase domain to the 3' portion of the Cas substrate domain (a.a. S³²⁵ to R⁵¹⁶). Src^KM is the isolated inactive kinase domain (K295M). Src^KM/Cas(5'SD) is a fusion of the inactive Src kinase domain to the 5' portion of the Cas substrate domain (a.a. L¹⁵⁷ to R⁵¹⁶). Src^KM/Cas(3'SD) is a fusion of the inactive Src kinase domain to the 3' portion of the Cas substrate domain (a. a. S³²⁵ to R⁵¹⁶).