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. 2002 Jul 16;3:20. doi: 10.1186/1471-2091-3-20

Table 3.

Kinetic parameters of hAps1, hAps1-39N and hAps2

Enzyme Substrate Km (μM) kcat (s-1) kcat/Km (103M-1s-1)
hAps1 Ap6A 13 ± 5 0.17 ± 0.04 13.1
Ap5A 37 ± 9 0.4 ± 0.1 10.8
PP-InsP5 (n = 2) 4 0.20 50
hAps1-39N Ap6A 11 ± 3 0.16 ± 0.02 14.5
Ap5A 50 ± 10 0.4 ± 0.1 8.0
PP-InsP5 (n = 2) 0.3 0.09 290
hAps2 Ap6A 19 ± 1 0.20 ± 0.01 10.5
Ap5A 50 ± 10 0.8 ± 0.2 16.0
PP-InsP5 1.3 ± 0.6 0.14 ± 0.02 110