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. 2025 Jan 29;20(1):e0317028. doi: 10.1371/journal.pone.0317028

The impact of Daylight Saving Time on dog activity

Lavania Nagendran 1,*,#, Ming Fei Li 2,#, David R Samson 1, Lauren Schroeder 1
Editor: Vinícius Silva Belo3
PMCID: PMC11778716  PMID: 39879143

Abstract

While most studies on Daylight Saving Time (DST) focus on human sleep and well-being, there is a dearth of understanding of how this sudden, human-mitigated change affects the routines of companion animals. The objective of this study was to assess how DST influenced the morning activity pattern of dogs (Canis familiaris). We used accelerometers to record activity in 25 sled dogs and 29 caregiver-companion dog dyads located in or near Ontario, Canada during the Fall Back time shift. We looked at morning onset activity based on sunrise time (both groups), handler arrival time (sled dogs), and caregiver Got-up Time (companion dogs), and compared pre-DST measures with the three days following DST. We found that sled dogs were less active around sunrise in all post-DST days compared to pre-DST, as sunrise coincided with handler arrival time before DST but not after. Companion dogs showed no change in morning activity based on sunrise times before and after DST. On the Sunday of DST, sled dogs were more active before handlers arrived, but their activity returned to pre-DST levels in the following two days. Caregiver and companion dog activities did not change on the day of DST. After DST, caregivers woke up earlier on weekdays, but companion dogs maintained their pre-DST activity patterns. Overall, we found that sled dogs took one day to adjust to a change in handler arrival time yet neither companion dogs nor their caregivers showed any morning activity difference after the Fall Back DST transition. In summary, our findings highlight the importance of flexible routines and gradual changes in helping dogs adjust to abrupt schedule modifications, offering valuable insights for optimizing dog care practices during time shifts.

Introduction

Daylight Saving Time (DST) occurs twice a year in some countries and is characterized by an hour change in local clock time primarily to align daylight hours with human activity periods [1]. In the Northern Hemisphere, the clock time is pushed forward in the spring between March and April (Spring Forward) and pushed back in the fall/autumn between September and November (Fall Back). Harrison’s [1] review of scientific literature examining the influence of DST on human sleep and behaviour suggests that disruptions in sleep continuity (e.g., increased sleep fragmentation and intra-daily variability) and efficiency may last up to a week after the shift. Individuals with extreme chronotypes, which would be an individual’s inclination to sleep much later or rise much earlier (extreme “evening” and morning” types, respectively), experienced greater difficulty adjusting to the time change. This was also observed among individuals who reported poor sleep quality prior to the time shift. In a longitudinal study spanning more than two decades, Varughese and Allen [2] found that increased fatal traffic accidents were reported following both DST shifts. The authors suggested that physiological adjustments relating to sleep deprivation resulted in higher fatalities in the spring, while the fatalities following the Fall Back transition were associated with behavioural changes anticipating the additional hour of sleep (e.g., staying out later the night before, driving drowsy or under the influence of alcohol). In summary, there is considerable evidence that DST has a substantial impact on human sleep and behaviour.

Recognizing that DST is an extreme form of anthropogenic intervention on the effects of natural light on circadian rhythm regulation [3, 4], we aim to investigate how this abrupt shift in the timing of human activity affects companion animals. This paper will be the first to examine how DST influences the activity of dogs (Canis familiaris). Although research on this topic is limited, inferences can be made based on what is known about dog activity patterns. Dogs are highly entrained to human behaviour. For instance, Griss and colleagues [5] found that the activity pattern of companion dogs was more correlated with that of their caregivers compared to free-ranging dogs. Furthermore, activity of kennel-housed sled dogs was primarily driven by human activity rather than environmental variables [6]. Many studies have also explored the relationship between dog and human sleep patterns through actigraphy measures [79]. Actigraphy studies on human-dog dyads that co-sleep (sleep in the same bed or bedroom) have shown dog activity to have a greater influence on human activity rather than human activity on dog activity [7, 8]. For example, Hoffman and colleagues [8] found that dog movement transitioning to human movement was more likely than human movement transitioning to dog movement, which suggests that human wake-ups are more dependent on dog activity. During nighttime, dogs were awake approximately three times longer than their caregivers, which may be attributed to their shorter sleep-wake cycles [8]. Taken together, these studies show that dog activity has a greater influence on human activity than vice versa, and that dog activity shows greater flexibility due to their short sleep cycles [10].

The aim of this study is to assess how readily dogs adjust to the Fall Back DST change in Canada. Given the high entrainment of dog and human activities, we hypothesize that dogs’ adjustment to DST will mirror their human counterparts. Specifically, we expect sled dogs, with their rigid schedules, to show significant shifts in morning activity patterns immediately after DST, similar to their human handlers. In contrast, companion dogs, with more flexible routines influenced by their caregivers’ schedules, are predicted to exhibit variable adjustment patterns. Additionally, we hypothesize that dogs with highly regulated routines will show a more pronounced alignment in their adjustment to DST. Therefore, sled dogs are anticipated to adjust more immediately and consistently compared to companion dogs. Alternatively, dogs may not show a consistent adjustment to DST, independent of their human counterparts. This would suggest no significant difference in adjustment patterns between sled dogs and companion dogs, indicating that factors beyond human-imposed schedules, such as individual temperament or environmental cues, are critical. Under this scenario, we expect high variation in dogs’ adjustment to DST, with no clear pattern of alignment with their human counterparts.

Methods

Data collection

This study includes two dog groups: sled dogs from Haliburton Forest & Wild Life Reserve in Haliburton, Ontario, Canada (45.22° N, 78.59° W); and companion dog and human caregiver dyads from Ontario and Quebec, Canada. Data were collected from 25 sled dogs (S1 Table) from October 25, 2020 to December 3, 2020 (clocks turned back one hour at 2:00 am on November 1, 2020). For background information on kennel setup, feeding, and activity schedules at Haliburton Forest & Wild Life Reserve, see S1 File.

For companion dog and caregiver dyads, human participants over the age of 18 years who lived in or near Ontario and who had either a husky or malamute dog were recruited for this study through poster advertising on social media and through word-of-mouth referrals. Husky and malamutes were chosen since these breeds are traced back to an ancient lineage of dogs originating from Zokhov Island, Siberia associated with sledding [11]. We also wanted to control for any breed-level differences in activity patterns [12]. Dogs over the age of 13 or who had prior medical issues that would influence their activity patterns were excluded from the study. Interested individuals who met the criteria and could commit to the study requirements took part in the study with their dogs. Data were collected from 37 companion dogs from November 2, 2021 to December 9, 2021 (clocks turned back one hour at 2:00 am on November 7, 2021).

One sled dog and eight companion dogs (and their associated human caregiver) were excluded from all analyses (see S1 Table). CAN017, CAN095 lost their sensors during the data collection period; the associated caregiver of CAN095, CAH037 was also excluded from the analyses. CAN065 and dyad CAN072-CAH024 were excluded due to incomplete data, meaning the sensor did not pick up activity for part of the data collection period. Five companion dog-caregiver dyads (CAN061-CAH016, CAN062-CAH017, CAN066-CAH020, CAN082-CAH032, CAN098-CAH040) were excluded because they did not have enough pretransition data (less than two days). Including individuals with less than two pre-transition dates would potentially skew data as a result of the weekend effect [13, 14]. Dyad CAN087-CAH034 were excluded since the caregiver did not make any changes to routines for DST. Our results are not expected to be affected by the exclusion.

The CamNtech MotionWatch 8 accelerometer was used to record human and dog activity. The accelerometer records and quantifies movement as acceleration waveforms per second [15]. We set the accelerometer to record activity for every 1-minute epoch (summation of MotionWatch counts over a 1-minute interval). For dogs, the accelerometer was fastened to a nylon collar with Gorilla Tape similar to the attachment protocol used for sled dogs in [6]. Human participants wore the accelerometer around their wrist and were instructed to wear it on their non-dominant hand. Once materials were prepped, the items were shipped to each participant. The recruitment process and interactions were done remotely as a result of the provincial lockdown measures placed due to the COVID-19 pandemic. Once materials were delivered, human participants wore the watch and placed collars on dogs and noted the date and time. Activity was recorded until the end of the data collection period at which point, caregivers removed the collars and watches and shipped the items back to researchers. The raw activity count was then downloaded using MotionWare software (version 1.2.23). Human participants provided demographic information and details about their schedule and relationship with their dogs as a part of a larger study. We obtained the sunrise and sunset times for each city from sunrise-sunset.org.

To determine human sleep measures, author MFL first cleaned the raw actigraphy data by deleting periods of non-wear times (i.e., when watch was taken off) that exceeded 30 minutes. We had asked participants to press an Event marker button to signal when they went to bed and got up and these markers were used to assist with scoring sleep periods. MFL visually determined the period between when participants went to bed (decrease in activity levels) and the “Got-up Time” which was when participants got out of bed (increase and consistency in activity levels). MotionWare then algorithmically categorized each 1-minute epoch as “sleep” or “wake” throughout the sleep period—this is how MotionWare designates “Woke-up Time” (sleep offset: when participants woke up) and this time always preceded the scored “Got-up Time” [15].

Ethics statement

Ethical approval was obtained for this study through the University of Toronto and written consent was obtained from all participating parties prior to data collection. Methods used for this study are in accordance with the guidelines and regulations in place by the University of Toronto Animal Care Committee (Protocol # 20012651) and the University of Toronto Human Research Ethics Board (Protocol # 40107). Methods were also reviewed and approved by staff at Haliburton Forest & Wild Life Reserve. This study complies with the Animal Research: Reporting of In Vivo Experiments (ARRIVE) guidelines [16]. Human participants received monetary compensation for their participation.

Data analyses

Sled dogs

To determine how long it took sled dogs to adjust to DST transition, we looked at two activity measures: morning onset activity according to handler arrival time (sum of activity between 6:31 am and 7:30 am, which was one hour before handlers arrived at 7:30 am), and morning onset activity according to sunrise time (sum of activity 30 minutes before and after sunrise). To calculate sled dogs’ activity measures pre-transition, we averaged activity measures across the seven days before DST transition (October 25 to October 31). We then compared the activity measure for each of the three consecutive days following DST transition to the pre-transition average (Pre). In other words, we compared DST1 (November 1) to Pre, DST2 (November 2) to Pre, and DST3 (November 3) to Pre. Both morning onset activity measures were not normally distributed so we used Wilcoxon signed rank tests. We also used a one-way repeated measures ANOVA to determine whether there were differences among the three post-transition dates. Again, since the morning onset activities were not normally distributed, we used the Friedman test. See S1 File for additional analyses the effect of DST on total daily activity (sum of activity over 24-hour period; S1 Fig) and the effects of sex and age on morning onset activities for sled dogs (S2 and S3 Tables).

Companion dogs

To determine how long it took companion dogs to adjust to DST transition, we looked at two activity measures: morning onset activity according to their caregiver’s Got-up Time (sum of activity 30 minutes before and after caregivers’ Got-up Time, as scored via MotionWare), and morning onset activity according to sunrise time (sum of activity 30 minutes before and after sunrise). To calculate companion dogs’ activity measures pre-transition, we averaged activity measures across the days before DST transition. Days pre-transition varied (ranged from 2 to 5 days; November 2 to November 6) depending on when participants received the study equipment and data collection began. We excluded five dyads (S1 Table) that only had one day of data pre-transition since this would have been a Saturday and dog activity is known to differ on weekends compared to weekdays [e.g., 15, 16]. Similar to the sled dog analyses, we then compared the activity measure for each of the three consecutive days following DST transition to the pre-transition measure (Pre). In other words, we compared DST1 (November 7) to Pre, DST2 (November 8) to Pre, and DST3 (November 9) to Pre. Both morning onset activity measures were not normally distributed so we used Wilcoxon signed rank tests. We also used the Friedman test to see whether there were differences among the three post-transition dates. See S1 File for additional analyses on total daily activity (S2 Fig) and the effects of sex, age, and presence of other dogs in the household on morning onset activities for companion dogs (S2 and S3 Tables).

Human caregivers

To determine how DST transition affected human sleep and activity, we compared caregiver Woke-up Time and Got-up Time for each of the three consecutive days following DST transition to the pre-transition measure (days were the same as for companion dogs). Both Woke-up Time and Got-up Time were normally distributed so we used paired t-tests. We also used a one-way repeated measures ANOVA to determine whether there were differences among the three post-transition dates.

All statistical analyses were performed in R Studio (v.2023.12.1+402; R v.4.0.1) for Mac OS X [17]. All statistical tests were two-tailed, with alpha set to 0.05 for significance.

Results

For sled dogs, mean morning onset activity according to handler arrival time was 3659.02 (± SD 2137.88) Pre-DST and 10983.04 (± SD 6026.74) on DST1. Mean morning onset activity according to sunrise time was 23844.95 (± SD 12596.34) Pre-DST and 7603.75 (± SD 5055.11) on DST1. For companion dogs, mean morning onset activity according to caregiver got-up time was 11951.21 (± SD 14310.27) Pre-DST and 12031.07 (± SD 16653.6) on DST 1. Mean morning onset activity according to sunrise time was 15023.58 (± SD 23189.57) Pre-DST and 5060.655 (± SD 5158.09) on DST1. See Table 1 for the complete descriptive statistics of all dog and human activity and sleep measures pre- and post-DST transition.

Table 1. Descriptive statistics of the mean (standard deviation) for morning onset activities in dogs and Woke-up and Got-up Times in human caregivers.

Sled Dogs Companion Dogs Human Caregivers
Date Morning activity—handler Morning activity—sunrise Morning activity—caregiver Morning activity—sunrise Woke-up Time (hh:mm) Got-up Time (hh:mm)
Pre-DST 3659.02 (2137.88) 23844.95 (12596.34) 11951.21 (14310.27) 15023.58 (23189.57) 7:46 am (1:11) 7:52 am (1:11)
DST1 10983.04 (6026.74) 7603.75 (5055.11) 12031.07 (16653.6) 5060.655 (5158.09) 8:03 am (2:20) 8:10 am (2:22)
DST2 4123.88 (5442.31) 4577.13 (5637.06) 12591.38 (16088.2) 15294.93 (27702.82) 6:50 am (1:11) 6:54 am (1:12)
DST3 4197.88 (4560.79) 4207.29 (4522.72) 16474.79 (30832.71) 25003.52 (58877.07) 7:09 am (1:14) 7:17 am (1:14)

Sled dogs

For morning onset activity based on handler arrival time, we found that only DST1 differed significantly from pre-transition (Table 2, Fig 1A and 1B). Dogs were significantly more active on DST1 than pre-transition. The Friedman test showed a significant difference among the three post-transition dates (χ2 = 25.33, p < 0.001). Nemenyi post hoc pairwise comparisons (with Bonferroni corrections) showed that dogs were significantly more active on DST1 than DST2 (p < 0.001) and DST3 (p < 0.001). However, there was no significant difference between DST2 and DST3.

Table 2. Results from pairwise comparisons of pre- and post-DST transition activity measures in sled dogs and companion dogs.

Sled Dog Analysis Companion Dog Analysis
Comparison Morning onset (handler) Morning onset (sunrise) Morning onset (caregiver) Morning onset (sunrise)
Pre–DST1 p < 0.001 p < 0.001 p = 0.508 p = 0.053
Pre–DST2 p = 0.527 p < 0.001 p = 0.831 p = 0.624
Pre–DST3 p = 0.966 p < 0.001 p = 0.865 p = 0.624

Significant effects (p < 0.05) are bolded.

Fig 1.

Fig 1

Box plots comparing sled dog morning onset activity based on handler arrival time (a, b) and sunrise time (c, d) for pre- and post-DST transition. Grey lines in the paired box plots (b, d) denote within-individual differences between pre-DST and the day of DST.

For morning onset activity based on sunrise time, we found that all three post-transition dates differed significantly from pre-transition (Table 2, Fig 1C and 1D). Dogs were less active in all three days following transition compared to pre-transition. The Friedman test showed a significant difference among the three post-transition dates (χ2 = 15.08, p < 0.001). Nemenyi post hoc tests (with Bonferroni corrections) showed that dogs were significantly more active on DST1 than DST2 (p = 0.017) and DST3 (p < 0.001). However, there was no significant difference between DST2 and DST3.

Companion dogs

For morning onset activity based on caregiver get-up times, we found no difference between pre-transition and the three post-transition dates (Table 2; Fig 2A and 2B). The Friedman test did not show any significant difference among the three post-transition dates (χ2 = 0.62, p = 0.733). Similarly, for morning onset activity based on sunrise time, we found no difference between pre-transition and the three post-transition dates (Table 2; Fig 2C and 2D). The Friedman test did not show any significant difference among the three post-transition dates (χ2 = 3.38, p = 0.185).

Fig 2.

Fig 2

Box plots comparing companion dog morning onset activity based on caregiver Got-up Time (a, b) and sunrise time (c, d) for pre- and post-DST transition. Grey lines in the paired box plots (b, d) denote within-individual differences between pre-DST and the day of DST.

Humans

We found no significant difference in caregiver Woke-up Time between DST1 and Pre (p = 0.585), however, Woke-up Times on DST2 (p < 0.001) and DST3 (p = 0.046) were significantly earlier than Pre (Fig 3A and 3B). We found no significant difference in Got-up Time between DST1 and Pre (p = 0.542) and between DST3 and Pre (p = 0.054); see Fig 3C and 3D. Got-up Times on DST2 (p < 0.001) was significantly earlier than Pre. The ANOVA indicated a significant difference among three post-transition dates for Woke-up Time (F = 7.49, p = 0.018) and Got-up Time (F = 8.03, p = 0.015). However, Wilcox post hoc pairwise comparisons (with Bonferroni corrections) did not show significant differences between any of the DST dates for Woke-up and Got-up Times.

Fig 3.

Fig 3

Box plots comparing caregiver Woke-up Time (a, b) and Got-Up Time (c, d) for pre- and post-DST transition. Grey lines in the paired box plots (b, d) denote within-individual differences between pre-DST and the day of DST.

Effect of age on dog morning activity

In the models examining the effects of age, sex, and presence of other dogs on dogs’ morning onset activity, the only significant effect we found was that age was negatively associated with sled dogs’ morning onset activity based on handler arrival time. Older sled dogs tended to be less active when handlers came into the kennel in the morning (β = -0.113, p = 0.012). In the models looking at the change in morning onset activity on DST1, the only significant effect we found was that age was negatively associated with the difference between companion dogs’ DST1 and Pre-DST morning onset activity based on caregiver got-up time. Older companion dogs had decreased morning activity on DST1 compared to Pre-DST (β = -2249.8, p = 0.028). Full model results are reported in S2 and S3 Tables.

Discussion

Here, we report how sled dogs and companion dogs in Canada adjusted to the Fall Back transition. In sled dogs, we found their morning onset activity based on handler arrival time to be significantly higher on DST Sunday compared to pre-transition, and resumed to pre-transition levels on DST2 and DST3. In other words, sled dogs took one day to adjust to the time shift. In companion dogs, we did not find evidence for any changes in morning onset activity following DST. Similarly, we did not find caregivers’ Woke-up and Got-up Times to change on DST Sunday. However, we did find that while caregivers woke up earlier on the weekdays after DST, dogs maintained their pre-transition morning activity levels. Morning activity in both groups of dogs did not appear to follow sunrise times. Each of these findings will be discussed below.

Effect of sunrise on dog morning activity

Companion dogs showed no significant difference in their morning activity around sunrise time pre- compared to post-DST transition. Survey responses indicate that most companion dogs slept indoors (one dog consistently slept outdoors, and five dogs occasionally slept outdoors). Conversely, in sled dogs, their morning onset activity based on sunrise time was markedly higher pre-DST transition compared to all three days after DST. Sunrise time pre-DST (~7:50 am) had greater overlap with handler arrival time (7:30 am) compared to sunrise time immediately following DST (~ 6:50 am)—dogs remained inactive around sunrise after DST since handler arrival time was much later than sunrise. It is important to note that activity was higher on the day of DST relative to DST2 and DST3 which reflects sled dogs taking one day to adjust to the delay in handler arrival time. In diurnal mammals, sleep-wake patterns are entrained to the light-dark cycle due to melatonin, the main sleep-promoting hormone, being produced during the dark period of the day [4]. Recently, it has been shown that endogenous cues, such as light, affect activity patterns in dogs under laboratory conditions [18, 19]. However, many studies have shown that the activity of companion dogs strongly aligns with human routines [13, 20, 21], perhaps more than endogenous environmental variables [6]. In our study, we found that companion dogs’ morning activity was not entrained to sunrise, and this was also the case in outdoor kennel-housed sled dogs whose activity adjusted according to handler arrival time rather than sunlight.

Sled dog activity shift

Sled dogs follow very rigid morning routines since handler arrival time is always 7:30 am (clock time). As such, sled dogs have daily schedules that are more regulated by humans than the companion dogs in our study who faced flexible caregiver work routines and the presence of multiple caregivers in the house (discussed below). Drug detector dogs that returned to work after an extended period of leave took one day to adjust (“first-day-back effect”) [10], which was what we observed in sled dogs adjusting to DST. Flexibility in activity patterns may be attributed to shorter sleep-wake cycles [10, 22]. We suspect there may also be a synchrony effect since sled dogs are all housed in the same sex-specific enclosure so when one individual exhibits morning onset activity, the group may follow suit. Adams and Johnson [23] found that group-housed dogs were able to differentiate between different auditory stimuli even while they were asleep. Dogs were more likely to respond to other dogs’ barking compared to anthropogenic noises. Circadian rhythm synchrony was found in cohabiting marmoset pairs [24] and mice [25]. Future studies should quantitatively assess activity synchrony among group-housed dogs.

Human activity and companion dogs

Our results indicate that DST did not affect Woke-up and Got-up Times in humans, therefore, it is not surprising that DST did not affect morning onset activity in companion dogs. It also does not appear that dogs’ routines are dictating human wake-up times (i.e., neither humans nor dogs woke up 1 hour earlier on DST Sunday to adjust for morning needs). Caregiver Woke-up Times were earlier on DST2 and DST3 than before the DST change. DST2 followed the same pattern in caregiver Got-up Times but DST 3 did not show an increase in activity compared to pre-transition dates. Since DST2 and DST3 were Monday and Tuesday, respectively, the pattern in Woke-up Times may be indicative of caregivers waking up earlier on workdays compared to the previous Sunday (DST1). Dogs, on the other hand, did not show this pattern of increased morning onset activity during weekdays after DST. Social jetlag [26], defined as the mismatch between the internal biological clock and the social time one has to adhere to (e.g., work schedule), has been found to be stronger in human caregivers than dogs [21].

The lack of tight coupling between dog and caregiver morning activity following DST could be due to several study limitations. First, dogs slept in varying proximity to their human caregiver, thus, sleeping distance could impact the likelihood of human disruptions on dog activity and dog disruptions on human activity [8]. Second, we did not take into consideration the influence of other caregivers in the household that might affect dog morning activity. Third, in this study group, there was considerable variability in caregivers’ work arrangements, especially in light of the COVID-19 pandemic, which required flexibility in work accommodations (working in-office, fully remote, and hybrid). Regardless, survey responses indicated that dogs were spending the majority of their time with humans irrespective of the primary caregiver’s working commitments (i.e., more than half of the dogs spent less than 5 hours alone daily), therefore dogs’ morning activity could have been entrained to other members of the household.

The findings from our study reflects dog response to the Fall Back DST change in or near Ontario, Canada and may not mirror other regions that have different dog keeping practices. Companion dogs in our study showed variability in sleeping location, feeding times, and daily schedules (e.g., walk times). In this field study, we did not alter the daily routines of dogs to collect their daily activity data; therefore, we could not control for environmental or situational factors such as extreme weather conditions or changes made to day-to-day schedules. Future research could implement a daily “activity log” for caregivers to note detailed information about their daily schedule.

Effects of age, sex, and presence of other dogs on morning activity

We found that older sled dogs generally had lower morning activity levels around handler arrival time than younger dogs. In addition, older companion dogs had lower activity on DST Sunday compared to pre-DST (S1 File)—this finding was unexpected and warrants further research that specifically looks at how older dogs respond to schedule changes. We recommend caregivers of older dogs to be especially mindful in implementing sudden changes to daily routines. The general trend of dog activity decreasing with age concurs with findings from other studies [e.g., 5, 16]. However, in a previous study with the same group of sled dogs, we did not find age to have an effect on total daytime activity [6]. Perhaps older dogs have decreased morning activity levels, or older dogs may be less reactive to human presence, compared to younger dogs. Interestingly, neither sex nor presence of other dogs in the household (i.e., among companion dogs) affected morning activity levels or the change in morning activity level following DST.

Conclusion

The significance of this research lies in its illumination of the intricate and bidirectional relationship between humans and their canine companions. This study underscores how human-imposed schedule changes, such as DST, can ripple through the daily lives of dogs, affecting their activity patterns and well-being. Understanding these effects is crucial for both dog owners and professionals working with dogs, as it can inform strategies to mitigate potential stressors associated with time changes. While the majority of literature examining the effect of DST on sleep and well-being has focused on humans, our paper is the first to quantify how this sudden, human-mitigated change affects the routines of domestic dogs. By recognizing the interconnectedness of human and dog activities, we can better appreciate the complexities of our shared environments and work towards fostering living conditions that support the health and well-being of both humans and their canine companions.

Supporting information

S1 Table. Summary of information for study participants.

Associated human ID is provided for companion dogs to note dyads. Information about breed, sex, age and weight is provided for canids. Age in years and weight in kilograms reflects that at time of data collection. Asterisks (*) next to ID notes individuals that were excluded for the study, see note below chart for more detailed reasoning.

(DOCX)

pone.0317028.s001.docx (22.3KB, docx)
S2 Table. Results from linear mixed-effects models on the effects of sex, age, and other dogs on morning onset activities for sled dogs and companion dogs.

(DOCX)

pone.0317028.s002.docx (15.3KB, docx)
S3 Table. Results from linear regressions on the effects of sex, age, and other dogs on morning onset activity difference between DST1 and pre-DST for sled dogs and companion dogs.

(DOCX)

pone.0317028.s003.docx (15.3KB, docx)
S1 Fig. Box plots comparing sled dog total daily activity for pre- and post-DST transition dates in sled dogs.

(TIF)

pone.0317028.s004.tif (266.7KB, tif)
S2 Fig. Box plots comparing companion dog total daily activity for pre- and post-DST transition dates in companion dogs.

(TIF)

pone.0317028.s005.tif (324.7KB, tif)
S1 File. Additional information on sled dogs, analyses on total daily activity, and analyses on the effects of age, sex, and presence of other dogs on morning activity.

(DOCX)

pone.0317028.s006.docx (19.5KB, docx)
S2 File. Dataset used to generate study results.

(XLSX)

pone.0317028.s007.xlsx (13.2MB, xlsx)

Acknowledgments

We are grateful for the participation of both dogs and caregivers for providing data for this study. This research would not be possible without the involvement of caregivers and staff at Haliburton Forest & Wild Life Reserve who provided background information and had the responsibility of equipment attachment.

Data Availability

All relevant data are within the manuscript and its Supporting Information files.

Funding Statement

This research was supported through Discovery Grants from the Natural Sciences and Engineering Research Council of Canada (RGPIN-2020-04159 to L.S. and RGPIN-2020-05942 to D.R.S).

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Decision Letter 0

Vinícius Silva Belo

11 Nov 2024

PONE-D-24-30341The impact of Daylight SavingTime on dog activityPLOS ONE

Dear Dr. Nagendran,

Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process.

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Vinícius Silva Belo

Academic Editor

PLOS ONE

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Please state what role the funders took in the study. If the funders had no role, please state: "The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript." If this statement is not correct you must amend it as needed. 

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Please update your Data Availability statement in the submission form accordingly.

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Data requests to a non-author institutional point of contact, such as a data access or ethics committee, helps guarantee long term stability and availability of data. Providing interested researchers with a durable point of contact ensures data will be accessible even if an author changes email addresses, institutions, or becomes unavailable to answer requests.

Before we proceed with your manuscript, please also provide non-author contact information (phone/email/hyperlink) for a data access committee, ethics committee, or other institutional body to which data requests may be sent. If no institutional body is available to respond to requests for your minimal data, please consider if there any institutional representatives who did not collaborate in the study, and are not listed as authors on the manuscript, who would be able to hold the data and respond to external requests for data access? If so, please provide their contact information (i.e., email address). Please also provide details on how you will ensure persistent or long-term data storage and availability.

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Data sharing should never compromise participant privacy. It is therefore not appropriate to publicly share personally identifiable data on human research participants. The following are examples of data that should not be shared:

-Name, initials, physical address

-Ages more specific than whole numbers

-Internet protocol (IP) address

-Specific dates (birth dates, death dates, examination dates, etc.)

-Contact information such as phone number or email address

-Location data

-ID numbers that seem specific (long numbers, include initials, titled “Hospital ID”) rather than random (small numbers in numerical order)

Data that are not directly identifying may also be inappropriate to share, as in combination they can become identifying. For example, data collected from a small group of participants, vulnerable populations, or private groups should not be shared if they involve indirect identifiers (such as sex, ethnicity, location, etc.) that may risk the identification of study participants.

Additional guidance on preparing raw data for publication can be found in our Data Policy (https://journals.plos.org/plosone/s/data-availability#loc-human-research-participant-data-and-other-sensitive-data) and in the following article: http://www.bmj.com/content/340/bmj.c181.long.

Please remove or anonymize all personal information (location), ensure that the data shared are in accordance with participant consent, and re-upload a fully anonymized data set. Please note that spreadsheet columns with personal information must be removed and not hidden as all hidden columns will appear in the published file.

6. Please review your reference list to ensure that it is complete and correct. If you have cited papers that have been retracted, please include the rationale for doing so in the manuscript text, or remove these references and replace them with relevant current references. Any changes to the reference list should be mentioned in the rebuttal letter that accompanies your revised manuscript. If you need to cite a retracted article, indicate the article’s retracted status in the References list and also include a citation and full reference for the retraction notice.

Additional Editor Comments:

In addition to the comments provided by the reviewer, the following points should be considered:

-The reasons for the exclusion of 8 dogs should be stated in the text and in more detail than in the supplementary material. In addition, possible effects of the exclusions should be discussed.

-The text should present information that can be highlighted from Table 1.

[Note: HTML markup is below. Please do not edit.]

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

**********

2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

**********

3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

**********

4. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

**********

5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: The study addresses a relevant topic: the influence of time changes, such as Daylight Saving Time, on dogs' routines, which can have significant implications for the animals' well-being and the routine of their guardian. The research used accelerometers to measure the dogs' activity, providing objective and quantitative data on their behavior during the time transition. If data are available, I suggest (but not as a requirement) that association tests be carried out between the change in behavior and some variables, such as: Dogs' age: Including an analysis by age can be a valuable addition, since different age groups have different activity levels and behavior patterns. Presence of other animals in the guardian's home: This analysis would allow us to observe whether interaction with other animals influences canine behavior during the time change. Where the animal is raised (indoors or outdoors): Considering this variable may be relevant, since living conditions (whether the animal lives indoors or outdoors) can impact both activity patterns and the dogs' adaptation to time changes. The inclusion of these variables could provide a broader view of the factors that influence dogs' behavior when faced with time changes, increasing the value and practical applicability of the research results.

**********

6. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #1: No

**********

[NOTE: If reviewer comments were submitted as an attachment file, they will be attached to this email and accessible via the submission site. Please log into your account, locate the manuscript record, and check for the action link "View Attachments". If this link does not appear, there are no attachment files.]

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PLoS One. 2025 Jan 29;20(1):e0317028. doi: 10.1371/journal.pone.0317028.r002

Author response to Decision Letter 0


1 Dec 2024

Thank you for the helpful comments. Please see the 'Response to Reviewers' document for responses in red. Pasted below are editor and review comments with authors' responses.

Journal Requirements:

When submitting your revision, we need you to address these additional requirements.

1. Please ensure that your manuscript meets PLOS ONE's style requirements, including those for file naming. The PLOS ONE style templates can be found at

https://journals.plos.org/plosone/s/file?id=wjVg/PLOSOne_formatting_sample_main_body.pdf and

https://journals.plos.org/plosone/s/file?id=ba62/PLOSOne_formatting_sample_title_authors_affiliations.pdf

We will rename all files according to PLOS ONE’s style requirements.

2. Thank you for stating the following financial disclosure:

“his research was supported through Discovery Grants from the Natural Sciences and Engineering Research Council of Canada (RGPIN-2020-04159 to L.S. and RGPIN-2020-05942 to D.R.S).”

Please state what role the funders took in the study. If the funders had no role, please state: "The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript." If this statement is not correct you must amend it as needed.

Please include this amended Role of Funder statement in your cover letter; we will change the online submission form on your behalf.

We have added this amended Role of Funder statement at the bottom of our revision cover letter.

3. We note that you have indicated that there are restrictions to data sharing for this study. For studies involving human research participant data or other sensitive data, we encourage authors to share de-identified or anonymized data. However, when data cannot be publicly shared for ethical reasons, we allow authors to make their data sets available upon request. For information on unacceptable data access restrictions, please see http://journals.plos.org/plosone/s/data-availability#loc-unacceptable-data-access-restrictions.

Before we proceed with your manuscript, please address the following prompts:

a) If there are ethical or legal restrictions on sharing a de-identified data set, please explain them in detail (e.g., data contain potentially identifying or sensitive patient information, data are owned by a third-party organization, etc.) and who has imposed them (e.g., a Research Ethics Committee or Institutional Review Board, etc.). Please also provide contact information for a data access committee, ethics committee, or other institutional body to which data requests may be sent.

b) If there are no restrictions, please upload the minimal anonymized data set necessary to replicate your study findings to a stable, public repository and provide us with the relevant URLs, DOIs, or accession numbers. Please see http://www.bmj.com/content/340/bmj.c181.long for guidelines on how to de-identify and prepare clinical data for publication. For a list of recommended repositories, please see https://journals.plos.org/plosone/s/recommended-repositories. You also have the option of uploading the data as Supporting Information files, but we would recommend depositing data directly to a data repository if possible.

Please update your Data Availability statement in the submission form accordingly.

We decided to upload the raw data used to generate the results of our study as a “Supporting Information” file. We have updated our Data Availability statement in the submission form.

4. In this instance it seems there may be acceptable restrictions in place that prevent the public sharing of your minimal data. However, in line with our goal of ensuring long-term data availability to all interested researchers, PLOS’ Data Policy states that authors cannot be the sole named individuals responsible for ensuring data access (http://journals.plos.org/plosone/s/data-availability#loc-acceptable-data-sharing-methods).

Data requests to a non-author institutional point of contact, such as a data access or ethics committee, helps guarantee long term stability and availability of data. Providing interested researchers with a durable point of contact ensures data will be accessible even if an author changes email addresses, institutions, or becomes unavailable to answer requests.

Before we proceed with your manuscript, please also provide non-author contact information (phone/email/hyperlink) for a data access committee, ethics committee, or other institutional body to which data requests may be sent. If no institutional body is available to respond to requests for your minimal data, please consider if there any institutional representatives who did not collaborate in the study, and are not listed as authors on the manuscript, who would be able to hold the data and respond to external requests for data access? If so, please provide their contact information (i.e., email address). Please also provide details on how you will ensure persistent or long-term data storage and availability.

Data was uploaded as a Supporting Information file. Please see the above comment.

5. We note that there is identifying data in the Supporting Information file <Supporting Information.docx>. Due to the inclusion of these potentially identifying data, we have removed this file from your file inventory. Prior to sharing human research participant data, authors should consult with an ethics committee to ensure data are shared in accordance with participant consent and all applicable local laws.

Data sharing should never compromise participant privacy. It is therefore not appropriate to publicly share personally identifiable data on human research participants. The following are examples of data that should not be shared:

-Name, initials, physical address

-Ages more specific than whole numbers

-Internet protocol (IP) address

-Specific dates (birth dates, death dates, examination dates, etc.)

-Contact information such as phone number or email address

-Location data

-ID numbers that seem specific (long numbers, include initials, titled “Hospital ID”) rather than random (small numbers in numerical order)

Data that are not directly identifying may also be inappropriate to share, as in combination they can become identifying. For example, data collected from a small group of participants, vulnerable populations, or private groups should not be shared if they involve indirect identifiers (such as sex, ethnicity, location, etc.) that may risk the identification of study participants.

Additional guidance on preparing raw data for publication can be found in our Data Policy (https://journals.plos.org/plosone/s/data-availability#loc-human-research-participant-data-and-other-sensitive-data) and in the following article: http://www.bmj.com/content/340/bmj.c181.long.

Please remove or anonymize all personal information (location), ensure that the data shared are in accordance with participant consent, and re-upload a fully anonymized data set. Please note that spreadsheet columns with personal information must be removed and not hidden as all hidden columns will appear in the published file.

We have removed “Location” from the Supporting Information file.

6. Please review your reference list to ensure that it is complete and correct. If you have cited papers that have been retracted, please include the rationale for doing so in the manuscript text, or remove these references and replace them with relevant current references. Any changes to the reference list should be mentioned in the rebuttal letter that accompanies your revised manuscript. If you need to cite a retracted article, indicate the article’s retracted status in the References list and also include a citation and full reference for the retraction notice.

We have reviewed our reference list and ensured that it is complete and correct. We did not add or remove any references.

Additional Editor Comments:

In addition to the comments provided by the reviewer, the following points should be considered:

-The reasons for the exclusion of 8 dogs should be stated in the text and in more detail than in the supplementary material. In addition, possible effects of the exclusions should be discussed.

We included the reason for the exclusion of 1 sled dog and 8 companion dogs, and that we do not expect these exclusions to change our study findings. This information was added to the manuscript text (lines 105-115).

-The text should present information that can be highlighted from Table 1.

We have added text to the main manuscript that highlights pertinent information from Table 1 (lines 195-201).

[Note: HTML markup is below. Please do not edit.]

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

________________________________________

2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

________________________________________

3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

________________________________________

4. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

________________________________________

5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: The study addresses a relevant topic: the influence of time changes, such as Daylight Saving Time, on dogs' routines, which can have significant implications for the animals' well-being and the routine of their guardian. The research used accelerometers to measure the dogs' activity, providing objective and quantitative data on their behavior during the time transition. If data are available, I suggest (but not as a requirement) that association tests be carried out between the change in behavior and some variables, such as: Dogs' age: Including an analysis by age can be a valuable addition, since different age groups have different activity levels and behavior patterns. Presence of other animals in the guardian's home: This analysis would allow us to observe whether interaction with other animals influences canine behavior during the time change. Where the animal is raised (indoors or outdoors): Considering this variable may be relevant, since living conditions (whether the animal lives indoors or outdoors) can impact both activity patterns and the dogs' adaptation to time changes. The inclusion of these variables could provide a broader view of the factors that influence dogs' behavior when faced with time changes, increasing the value and practical applicability of the research results.

We thank the reviewer for these helpful suggestions. We agree that these additional analyses would provide further insight on dogs’ morning behavior and how they may respond to time changes. For sled dogs, we included additional analyses on how sex and age affect general morning activity levels and the change in morning activity on DST1 compared to Pre-DST. We did not include the presence of other dogs since all sled dogs live in kennels with other dogs and all kennels are located close to each other and are outdoors. For companion dogs, we included additional analyses on how sex, age, and presence of other dogs in the household affect general morning activity levels and the change in morning activity on DST1 compared to Pre-DST. We did not include location since all companion dogs, except for four, slept indoors during the study period. The four dogs that sometimes slept outside were all from the same household. Our main findings were that, compared to younger dogs, older sled dogs had lower morning onset activity around handler arrival time, and older companion dogs had lower morning onset activity based on caregiver got-up time on DST1 compared to Pre-DST.

We included the additional analyses and results in the Supporting Information file as well as in the main manuscript (lines 165-166, 183-184, 251-260). We also added an additional section to our Discussion interpreting results from these additional analyses (lines 336-348).

________________________________________

6. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #1: No

________________________________________

Attachment

Submitted filename: Response to Reviewers.docx

pone.0317028.s008.docx (293.4KB, docx)

Decision Letter 1

Vinícius Silva Belo

20 Dec 2024

The impact of Daylight SavingTime on dog activity

PONE-D-24-30341R1

Dear Dr. Nagendran,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

An invoice will be generated when your article is formally accepted. Please note, if your institution has a publishing partnership with PLOS and your article meets the relevant criteria, all or part of your publication costs will be covered. Please make sure your user information is up-to-date by logging into Editorial Manager at Editorial Manager® and clicking the ‘Update My Information' link at the top of the page. If you have any questions relating to publication charges, please contact our Author Billing department directly at authorbilling@plos.org.

If your institution or institutions have a press office, please notify them about your upcoming paper to help maximize its impact. If they’ll be preparing press materials, please inform our press team as soon as possible -- no later than 48 hours after receiving the formal acceptance. Your manuscript will remain under strict press embargo until 2 pm Eastern Time on the date of publication. For more information, please contact onepress@plos.org.

Kind regards,

Vinícius Silva Belo

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #1: All comments have been addressed

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The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

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3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

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4. Have the authors made all data underlying the findings in their manuscript fully available?

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Reviewer #1: Yes

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5. Is the manuscript presented in an intelligible fashion and written in standard English?

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Reviewer #1: Yes

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6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: (No Response)

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Reviewer #1: Yes: PAULO HENRIQUE ARAÚJO SOARES

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Acceptance letter

Vinícius Silva Belo

26 Dec 2024

PONE-D-24-30341R1

PLOS ONE

Dear Dr. Nagendran,

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on behalf of

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Academic Editor

PLOS ONE

Associated Data

    This section collects any data citations, data availability statements, or supplementary materials included in this article.

    Supplementary Materials

    S1 Table. Summary of information for study participants.

    Associated human ID is provided for companion dogs to note dyads. Information about breed, sex, age and weight is provided for canids. Age in years and weight in kilograms reflects that at time of data collection. Asterisks (*) next to ID notes individuals that were excluded for the study, see note below chart for more detailed reasoning.

    (DOCX)

    pone.0317028.s001.docx (22.3KB, docx)
    S2 Table. Results from linear mixed-effects models on the effects of sex, age, and other dogs on morning onset activities for sled dogs and companion dogs.

    (DOCX)

    pone.0317028.s002.docx (15.3KB, docx)
    S3 Table. Results from linear regressions on the effects of sex, age, and other dogs on morning onset activity difference between DST1 and pre-DST for sled dogs and companion dogs.

    (DOCX)

    pone.0317028.s003.docx (15.3KB, docx)
    S1 Fig. Box plots comparing sled dog total daily activity for pre- and post-DST transition dates in sled dogs.

    (TIF)

    pone.0317028.s004.tif (266.7KB, tif)
    S2 Fig. Box plots comparing companion dog total daily activity for pre- and post-DST transition dates in companion dogs.

    (TIF)

    pone.0317028.s005.tif (324.7KB, tif)
    S1 File. Additional information on sled dogs, analyses on total daily activity, and analyses on the effects of age, sex, and presence of other dogs on morning activity.

    (DOCX)

    pone.0317028.s006.docx (19.5KB, docx)
    S2 File. Dataset used to generate study results.

    (XLSX)

    pone.0317028.s007.xlsx (13.2MB, xlsx)
    Attachment

    Submitted filename: Response to Reviewers.docx

    pone.0317028.s008.docx (293.4KB, docx)

    Data Availability Statement

    All relevant data are within the manuscript and its Supporting Information files.


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