Table 1.
Whole Embryo | Heart | Notochord | |||||||||
hpf | mean | SEM | p-value | hpf | mean | SEM | p-value | hpf | mean | SEM | p-value |
8–24 | -1.24 | 2.25 | 0.37 | 8–24 | 8–24 | 0.58 | 4.51 | 0.25 | |||
8–32 | 8.40 | 3.61 | 0.07 | 8–32 | 8–32 | 16.07 | 5.60 | 0.03 | |||
8–56 | 11.59 | 3.66 | 0.003 | 8–56 | 9.41 | 8.12 | 0.17 | 8–56 | 10.11 | 6.23 | 0.08 |
24–48 | 8.81 | 2.86 | 0.01 | 24–48 | 12.19 | 5.83 | 0.04 | 24–48 | 16.17 | 4.56 | 0.003 |
24–72 | 18.62 | 4.58 | 0.0004 | 24–72 | 27.75 | 4.82 | 0.00003 | 24–72 | 31.68 | 6.80 | 0.0001 |
24–80 | 10.07 | 5.72 | 0.11 | 24–80 | 11.15 | 6.44 | 0.13 | 24–80 | 17.77 | 8.11 | 0.06 |
32–56 | 2.67 | 5.19 | 0.29 | 32–56 | 35.60 | 12.44 | 0.02 | 32–56 | 2.25 | 7.29 | 0.32 |
32–80 | 1.21 | 4.14 | 0.43 | 32–80 | 0.38 | 5.97 | 0.48 | 32–80 | 3.91 | 7.17 | 0.35 |
48–72 | 1.97 | 2.50 | 0.28 | 48–72 | 8.42 | 3.96 | 0.04 | 48–72 | 4.13 | 4.51 | 0.22 |
56–80 | -2.58 | 2.82 | 0.27 | 56–80 | 5.01 | 5.61 | 0.27 | 56–80 | -7.11 | 4.35 | 0.15 |
Rohon Beard Neurons | Eye | Lens | |||||||||
hpf | mean | SEM | p-value | hpf | mean | SEM | p-value | hpf | mean | SEM | p-value |
8–24 | 8–24 | 2.07 | 4.80 | 0.40 | 8–24 | ||||||
8–32 | 8–32 | 3.01 | 5.23 | 0.36 | 8–32 | ||||||
8–56 | 9.65 | 5.84 | 0.08 | 8–56 | 14.06 | 6.73 | 0.04 | 8–56 | 17.73 | 6.69 | 0.01 |
24–48 | 8.05 | 4.65 | 0.09 | 24–48 | 12.65 | 4.57 | 0.01 | 24–48 | 10.75 | 4.74 | 0.03 |
24–72 | 17.29 | 5.08 | 0.01 | 24–72 | 15.25 | 7.18 | 0.03 | 24–72 | 18.55 | 7.28 | 0.02 |
24–80 | 14.36 | 7.28 | 0.09 | 24–80 | 11.27 | 7.78 | 0.15 | 24–80 | 10.41 | 7.78 | 0.18 |
32–56 | 1.90 | 6.78 | 0.40 | 32–56 | 8.96 | 8.58 | 0.18 | 32–56 | 11.73 | 9.13 | 0.14 |
32–80 | -1.60 | 5.72 | 0.41 | 32–80 | 0.70 | 5.94 | 0.47 | 32–80 | -4.09 | 5.57 | 0.34 |
48–72 | 5.53 | 4.10 | 0.17 | 48–72 | -3.77 | 3.73 | 0.24 | 48–72 | -4.73 | 4.52 | 0.22 |
56–80 | -4.76 | 4.30 | 0.24 | 56–80 | 1.25 | 5.06 | 0.44 | 56–80 | 2.83 | 5.91 | 0.37 |
Somites | |||||||||||
hpf | mean | SEM | p-value | ||||||||
8–24 | 4.28 | 3.38 | 0.23 | ||||||||
8–32 | 4.10 | 5.71 | 0.31 | ||||||||
8–56 | 7.07 | 5.25 | 0.13 | ||||||||
24–48 | 7.50 | 4.52 | 0.09 | ||||||||
24–72 | 22.24 | 6.30 | 0.002 | ||||||||
24–80 | 15.39 | 7.80 | 0.08 | ||||||||
32–56 | 3.11 | 7.17 | 0.32 | ||||||||
32–80 | 2.08 | 5.89 | 0.41 | ||||||||
48–72 | 8.24 | 6.52 | 0.12 | ||||||||
56–80 | -6.88 | 3.50 | 0.13 |
hpf = hours post-fertilization that the embryos were kept in the bioreactor (see Figure 2);
SEM = standard error of the mean.