Table 4.

SAG sequence variations and amino acid exchanges in respective dog breeds

			Sequence variations in breed(s)a
Location	Sequence variation*	Amino acid exchange	In affected and gPRA unaffected dogs	gPRA affected dogs in heterozygous state
Intron 1	IVS1+393T→C	-	BDP, BS, GR, IRS, Rob, TT	BDP, GR,
Exon 2	UTR-5A→G	-	Bo, BS, GRc, BDP, IRS, SDc, Sl, TTb	Bo, GR, SD
	c255A→G	H14C	SI	Sl
Exon 5	c526C→T	A111V	Sac	Sa
	c531G→A	A113T	Sac	Sa
Intron 5	IVS5-30C→T	-	ECS, MP, Rob, Sac	ECS, MP, Sa
Exon 6	c610C→T	C132C	GR	GR
Intron 7	IVS7+10C→T	-	IRSb, GR, SPc	D, IRS, GR
	IVS7+52Ins.G	-	GRc	GR
	IVS7-4Del.G	-	CCRb D, GR, IRS, MP, SDc, Slc, SPb, TTc	D, GR, IRS, SD
Exon 8	c811G→A	Q199Q	GR	GR
Intron 8	IVS8+8A→G	-	D, ECSc, GRc, LRc, Sa	D, ECS, GR, Sa
Exon 9	c874T→C	H225H	Bo, BDP, CCRb, ECS, D, GRc, IRS, MP, NF, Rob, Sa, Slb, TTbc	Bo, ECS, D, GR, IRS, MP, NF, Sa
Exon 10	c949G→T	V247V	IRS, GR	GR
	c983G→T	D259T	ACb	AC
	c1000A→G	P264P	AC, LR, IRSb, GR	GR
Intron 10	IVS10-18G→C	-	GR, IRS, BDP	GR
	IVS10-33C→T	-	GR, IRS	GR
Exon 11	c1063G→A	T288T	BS, BDP, GR, IRSb, Sa	BS, GR, Sa
	c1076C→T	L293L	BS, BDP, GRc, Sa, TT	BS, Sa
Intron 11	IVS11-51Ins.TT	-	Sa, IRSb	Sa
Intron 13	IVS13+66C→G	-	Sa, IRSb	Sa
Intron 14	IVS14-45Del.A	-	PON	PON
Intron 15	IVS15+14C→T	-	Sa	-
	IVS15+86T→A	-	Sa	-
	IVS15-45Ins.C	-	PON, AWbc, SPb	PON
Exon 16	c1344C→A	A379E	IRSb, ECSb, GRc, PON, Sabc, TTb	ECS, GR

^a For abbreviations see Table 1^b Homozygous state in gPRA affected dogs ^c Homozygous state in unaffected dogs ^*(EMBL accession number for cDNA: X98460; EMBL accession numbers for genomic DNA: AJ426068-AJ426078)