Figure 3.

Purification of PabI. The purification steps were monitored by SDS–PAGE electrophoresis. Marker, the protein size markers [Prestained SDS–PAGE Standards Low Range (BIO-RAD)]; total, wheat-germ-based cell-free protein synthesis solution containing expressed PabI; heated sup., the protein synthesis solution after its treatment at 90°C and low-speed centrifugation; purified PabI, the heated supernatant after Heparin–Sepharose column chromatography.