Figure 6.

Thermoresistance of PabI. (A) DNA cleavage activity of PabI at different temperatures. Linearized pKI1 was cleaved with PabI for 1 h at the specified temperature. RsaI: DNA cleaved with RsaI. PabI is active at temperatures ranging from 60 to 90°C. Although its activity increased at temperatures ranging from 60 to 85°C, the substrates and product DNAs seem to be altered at higher temperatures (90 and 95°C). (B) Heat resistance of PabI at 85°C. PabI was pre-heated at 85°C for the indicated duration and then used to cleave linearized pKI1 at 85°C for 1 h. The DNA products were separated through a 1% agarose gel and visualized under UV light after ethidium bromide staining. RsaI: DNA cleaved with RsaI.