Figure 1.

Plasmid R-M tests for Eco394I on a series of subclones identifying two Eco394I sites (A and B) on plasmid pL9. Positive plasmids (with Eco394I site) are darkened, whereas negative plasmids (no site) are hatched. Plasmid pL9 is a subclone of pL2 (Table 1). Plasmids pL31 to pL35 are PstI subclones of pL9. Plasmids pL49, pL50 and pL51 are derived from pL32, whereas plasmids pL54, pL55 and pL57 are derived from pL34. Two plasmids pL34-96 and pL34-97 were obtained from an ExoIII/S1 nested deletion experiment, whereas the pC2 is a plasmid containing an 18 bp oligonucleotide cloned into the pMECA EcoRV site. Only relevant DNA sequences are shown. Note that the Eco394I sequence described here at B site is a complementary sequence of the consensus sequence, GAC(5N)RTAAY described in Table 2.