Table 5.
Type I enzymes prototype recognition sequences
| Type I enzyme | Recognition sequencea | Pattern (total bp) | A-T distance | Familyb | Reference |
|---|---|---|---|---|---|
| Natural | |||||
| CfrAI | GCANNNNNNNNGTGG | 3-8-4 (15) | 9 | B | (10) |
| Eco377I | GGANNNNNNNNATGC | 3-8-4 (15) | 9 | – | (5) |
| Eco394I | GACNNNNNRTAAY | 3-5-5 (13) | 7 | – | This work |
| Eco585I | GCCNNNNNNTGCG | 3-6-4 (13) | – | – | (5) |
| Eco646I | CCANNNNNNNCTTC | 3-7-4 (14) | 8 | – | (5) |
| Eco777I | GGANNNNNNTATC | 3-6-4 (13) | 6 | – | (5) |
| Eco851I | GTCANNNNNNTGAY | 4-6-4 (14) | 6 | – | This work |
| Eco826I | GCANNNNNNCTGA | 3-6-4 (13) | 7 | – | This work |
| Eco912I | CACNNNNNTGGC | 3-5-4 (12) | 6 | – | This work |
| EcoAI | GAGNNNNNNNGTCA | 3-7-4 (14) | 9 | B | (11) |
| EcoBI | TGANNNNNNNNTGCT | 3-8-4 (15) | 8 | A | (12) |
| EcoDI | TTANNNNNNNGTCY | 3-7-4 (14) | 8 | A | (11;13) |
| EcoDXXI | TCANNNNNNNRTTC | 3-7-4 (14) | 8 | C | (14) |
| EcoEI | GAGNNNNNNNATGC | 3-7-4 (14) | 9 | B | (15) |
| EcoKI | AACNNNNNNGTGC | 3-6-4 (13) | 8 | A | (16) |
| EcoR124I | GAANNNNNNRTCG | 3-6-4 (13) | 7 | C | (17) |
| EcoR124II | GAANNNNNNNRTCG | 3-7-4 (14) | 8 | C | (17) |
| EcoprrI | CCANNNNNNNRTGC | 3-7-4 (14) | 8 | C | (18) |
| KpnAI | GAANNNNNNTGCC | 3-6-4 (13) | 6 | D | (7) |
| KpnBI | CAAANNNNNNRTCA | 4-6-4 (14) | 7 | E | (8) |
| NgoAV | GCANNNNNNNNTGC | 3-8-3 (14) | 8 | C | (19) |
| StyLTIII | GAGNNNNNNRTAYG | 3-6-4 (13) | 8 | A | (18;20) |
| StySEAI | ACANNNNNNTYCA | 3-6-4 (13) | 6 | – | (7) |
| StySBLI | CGANNNNNNTACC | 3-6-4 (13) | 6 | D | (21) |
| StySGI | TAANNNNNNRTCG | 3-6-4 (13) | 7 | – | (7) |
| StySKI | CGATNNNNNNNGTTA | 3-7-4 (14) | 9 | – | (7) |
| StySPI | AACNNNNNNGTRC | 3-6-4 (13) | 8 | A | (18;20) |
| Artificial constructs | |||||
| EcoDR2 | TCANNNNNNGTCG | 3-6-4 (13) | 7 | C | (22) |
| EcoDR3 | TCANNNNNNNATCG | 3-7-4 (14) | 8 | C | (22) |
| EcoRD2 | GAANNNNNNRTTC | 3-6-4 (13) | 7 | C | (22) |
| EcoRD3 | GAANNNNNNNRTTC | 3-7-4 (14) | 8 | C | (22) |
| EcoDXXIΔ | TCANNNNNNNNTGA | 3-8-3 (14) | 8 | C | (23) |
| Eco124IΔ | GAANNNNNNNTTC | 3-7-3 (13) | 7 | C | (24) |
| StySJI | GAGNNNNNNGTRC | 3-6-4 (13) | 8 | A | (25) |
| StySQI | AACNNNNNNRTAYG | 3-6-5 (14) | 8 | A | (26) |
aThe modified adenine is not experimentally verified for CfrAI, EcoEI and StySBLI, but each has only one candidate adenines in their recognition sequences.
bFamily yet to be determined.