Table 2. Coverage of EC prediction by iCSA filter.

	Swiss-Prot homologues
psi-blast iteration	Identified by 482 queries	With same EC as query	With same EC as query and correct iCSA filter assignment	Coverage, %
1	17,450	15,966	13,792	86.4
2	3,882	2,029	764	37.7
3	1,483	657	125	19.0
4	961	388	62	16.0
Overall	23,776	19,040	14,743	77.4

Results are shown for the iCSA prediction of EC number for Swiss-Prot homologues detected at each psi-blast iteration, up to four, using 482 nonredundant CSA enzymes as queries. “Overall” figures use the results from all four iterations in one calculation. EC predictions are deemed correct if they match to the third digit of the EC annotation in the Swiss-Prot database. Coverage is calculated as the number of homologues correctly assigned the same EC by iCSA divided by the number with the same EC as the queries. The figures given are for a nonredundant set of Swiss-Prot homologues (i.e., each Swiss-Prot appears only once because we need to record only whether an assignment has or has not been made in order to calculate coverage).